MAP2K2 Antibody (S226)
Référence MBS9217161-5x0,4mL
Conditionnement : 5x0.4mL
Marque : MyBiosource
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All lanes : Anti-MAP2K2-pS226. ctrl at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: MOLT-4 whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 44 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 1: Hela whole cell lysate
Lane 2: MOLT-4 whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 44 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
MAP2K2 Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9217161Description: MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3.… More details
Product Overview
Online Datasheet in PDFProduct Name
MAP2K2, Polyclonal Antibody
Full Product Name
MAP2K2 Antibody (S226)
Product Gene Name
anti-MAP2K2 antibody
Product Synonym Gene Name
MEK2; MKK2; PRKMK2
Product Synonym Names
Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2; MAP2K2; MEK2; MKK2; PRKMK2
Clonality
Polyclonal
Isotype
Ig
Host
Rabbit
Species Reactivity
Human; Predicted Reactivity: Drosophila, C.Elegans, Hamster, Mouse, Rabbit, Rat, Xenopus, Chicken
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Specificity
This MAP2K2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-233 amino acids from human MAP2K2.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
MAP2K2 (S226) peptide (MBS9227417) is used for blocking the activity of MAP2K2 antibody (MBS9217161)
Applications Tested/Suitable for anti-MAP2K2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-MAP2K2 antibody
WB:~~1:1000
Western Blot (WB) of anti-MAP2K2 antibody
All lanes : Anti-MAP2K2-pS226. ctrl at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: MOLT-4 whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 44 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 1: Hela whole cell lysate
Lane 2: MOLT-4 whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 44 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Product References and Citations for anti-MAP2K2 antibody
Burroughs, K.D., et al., Mol. Cancer Res. 1 (4):312-322 (2003). Tran, H., et al., Mol. Cell. Biol. 23 (20):7177-7188 (2003). Li, S.P., et al., Cancer Res. 63 (13):3473-3477 (2003). Li, Y., et al., J. Biol. Chem. 278 (16):13663-13671 (2003). Liu, X., et al., J. Biol. Chem. 277 (42):39312-39319 (2002).
Other Notes
Small volumes of anti-MAP2K2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MAP2K2 purchase
MBS9217161 is an antibody targeting MAP2K2. Also known as MAP2K2 Antibody (S226). Reactive in Human; Predicted Reactivity: Drosophila, C.Elegans, Hamster, Mouse, Rabbit, Rat, Xenopus, Chicken. Host: Rabbit.
Additional Details
NCBI/Uniprot data below describe general gene information for MAP2K2. It may not necessarily be applicable to this product.
NCBI Official Full Name
dual specificity mitogen-activated protein kinase kinase 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 2
NCBI Official Symbol
MAP2K2
NCBI Official Synonym Symbols
CFC4; MEK2; MKK2; MAPKK2; PRKMK2
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 2
UniProt Synonym Protein Names
ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2
Protein Family
Dual specificity mitogen-activated protein kinase kinase
UniProt Gene Name
MAP2K2
UniProt Synonym Gene Names
MEK2; MKK2; PRKMK2; MAP kinase kinase 2; MAPKK 2; MEK 2
UniProt Entry Name
MP2K2_HUMAN
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 2
NCBI Summary for MAP2K2
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for MAP2K2
MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction.
Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: cell cortex; cytoplasm; cytosol; early endosome; endoplasmic reticulum; extracellular region; focal adhesion; Golgi apparatus; intercellular junction; internal side of plasma membrane; late endosome; microtubule; mitochondrion; nucleus; perinuclear region of cytoplasm; peroxisomal membrane
Molecular Function: ATP binding; MAP kinase kinase activity; metal ion binding; PDZ domain binding; protein binding; protein complex scaffold; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein-tyrosine kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: activation of MAPK activity; heart development; MAPKKK cascade; peptidyl-tyrosine phosphorylation; positive regulation of axonogenesis; regulation of axon regeneration; regulation of stress-activated MAPK cascade; thymus development; thyroid gland development
Disease: Cardiofaciocutaneous Syndrome 4
Show more Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: cell cortex; cytoplasm; cytosol; early endosome; endoplasmic reticulum; extracellular region; focal adhesion; Golgi apparatus; intercellular junction; internal side of plasma membrane; late endosome; microtubule; mitochondrion; nucleus; perinuclear region of cytoplasm; peroxisomal membrane
Molecular Function: ATP binding; MAP kinase kinase activity; metal ion binding; PDZ domain binding; protein binding; protein complex scaffold; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein-tyrosine kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: activation of MAPK activity; heart development; MAPKKK cascade; peptidyl-tyrosine phosphorylation; positive regulation of axonogenesis; regulation of axon regeneration; regulation of stress-activated MAPK cascade; thymus development; thyroid gland development
Disease: Cardiofaciocutaneous Syndrome 4
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-MAP2K2 antibody
| Reference Product | PubMed Publications |
|---|---|
| MAPK3 antibody | >147 publications with MAP2K2 and MAPK3 |
| MAPK1 antibody | >146 publications with MAP2K2 and MAPK1 |
| BRAF antibody | >57 publications with MAP2K2 and BRAF |
| RAF1 antibody | >14 publications with MAP2K2 and RAF1 |
| MAP3K1 antibody | >8 publications with MAP2K2 and MAP3K1 |
| MAP3K8 antibody | >1 publications with MAP2K2 and MAP3K8 |
Pathways associated with anti-MAP2K2 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| ARMS-mediated Activation Pathway antibodies | ARMS-mediated Activation Pathway Diagram |
| Activated TLR4 Signalling Pathway antibodies | Activated TLR4 Signalling Pathway Diagram |
| Acute Myeloid Leukemia Pathway antibodies | Acute Myeloid Leukemia Pathway Diagram |
| Acute Myeloid Leukemia Pathway antibodies | Acute Myeloid Leukemia Pathway Diagram |
| Axon Guidance Pathway antibodies | Axon Guidance Pathway Diagram |
| B Cell Receptor Signaling Pathway antibodies | B Cell Receptor Signaling Pathway Diagram |
| B Cell Receptor Signaling Pathway antibodies | B Cell Receptor Signaling Pathway Diagram |
| B Cell Receptor Signaling Pathway antibodies | B Cell Receptor Signaling Pathway Diagram |
| BDNF Signaling Pathway antibodies | BDNF Signaling Pathway Diagram |
| Bladder Cancer Pathway antibodies | Bladder Cancer Pathway Diagram |
Diseases associated with anti-MAP2K2 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >166 publications with MAP2K2 and Neoplasms |
| Cardiovascular Diseases Antibodies | >37 publications with MAP2K2 and Cardiovascular Diseases |
| Heart Diseases Antibodies | >28 publications with MAP2K2 and Heart Diseases |
| Necrosis Antibodies | >26 publications with MAP2K2 and Necrosis |
| Inflammation Antibodies | >24 publications with MAP2K2 and Inflammation |
| Melanoma Antibodies | >24 publications with MAP2K2 and Melanoma |
| Nervous System Diseases Antibodies | >21 publications with MAP2K2 and Nervous System Diseases |
| Drug Toxicity Antibodies | >21 publications with MAP2K2 and Drug Toxicity |
| Breast Neoplasms Antibodies | >19 publications with MAP2K2 and Breast Neoplasms |
| Neoplasm Metastasis Antibodies | >14 publications with MAP2K2 and Neoplasm Metastasis |
Organs/Tissues associated with anti-MAP2K2 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >78 publications with MAP2K2 and Blood |
| Heart Antibodies | >34 publications with MAP2K2 and Heart |
| Skin Antibodies | >31 publications with MAP2K2 and Skin |
| Lung Antibodies | >31 publications with MAP2K2 and Lung |
| Brain Antibodies | >25 publications with MAP2K2 and Brain |
| Kidney Antibodies | >17 publications with MAP2K2 and Kidney |
| Liver Antibodies | >17 publications with MAP2K2 and Liver |
| Bone Antibodies | >16 publications with MAP2K2 and Bone |
| Muscle Antibodies | >11 publications with MAP2K2 and Muscle |
| Prostate Antibodies | >10 publications with MAP2K2 and Prostate |