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Monkeypox Virus A29L Protein Human IgM ELISA Kit Standard Curve
Monkeypox Virus A29L Protein Human IgM ELISA Kit
The MPOXA29 protein Human IgG/M ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgG or M antibody against MPOXA29 protein in human serum or plasma.
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human, Monkeypox Virus |
| Detection Range | 1.37 Units/mL - 1000 Units/mL |
| Compatible Sample Types | Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 1,500-fold |
| Gene Symbols | IGM |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Indirect |
| Method Of Detection | Colorimetric |
| Research Area | Infectious Disease, Mpox |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | -80°C |
Introduction
This MPOX human IgG/M antibody ELISA kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with the MPOXA29 protein, which combines with the corresponding antibody present in a sample and Positive Control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgG/M antibody is added, a complex form between biotinylated secondary antibody and human IgG/M antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the anti-A29 IgG/M antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Control consists of an anti-A29 antibody. A biotinylated anti-Positive Control antibody is added to the Positive Control wells and treated identically to the samples. The intensity of the resulting color (measured at 450 nm) is used as a calibration curve for interpretation purposes in the different assays.
Kit Components
| Component | Size / Description |
|---|---|
| MPOXA29 Protein Coated 96-Well Microplate | 96 wells coated with MPOXA29 protein |
| Wash Buffer | 25 mL |
| Positive Control | 1 vial |
| Biotinylated Anti-Human IgG/M | 1 vial |
| Biotinylated Anti-Positive Control | 1 vial |
| HRP-Streptavidin | 1 vial |
| TMB One-Step Substrate Reagent | 12 mL |
| Stop Solution | 8 mL |
| Assay Diluent | 15 mL |
| Sample Diluent | 25 mL |
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed.
- Add 100 µL positive control, or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µL prepared Biotinylated Anti-Positive Control and Biotinylated Anti-Human IgG/M Antibody into respective wells. Incubate 1 hour at room temperature.
- Add 100 µL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
- Add 50 µL Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
