Mouse CD44/Pgp-1 Antibody : LE/AF
Référence OASB00228
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Mouse CD44/Pgp-1 Antibody : LE/AF (OASB00228)
| Datasheets/Manuals | Printable datasheet for Mouse CD44/Pgp-1 Antibody : LE/AF (OASB00228) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | KM201 |
| Isotype | IgG1 |
| Host | Rat |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-F, IHC-P, ICC, IP, WB, BL |
| Additional Information | Description: CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal clone BMS2 |
| Concentration | 0.5 mg/mL |
| Specificity | CD44 |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested to conform to characteristics of astandard reference by flow cytometry. Representative data are included in this technical bulletin. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate <= 2 ug/106 cells Biotin conjugate <= 2 ug/106 cells R-phycoerythrin conjugate <= 0.2 ug/106 cells Allophycocyanin conjugate <= 0.2 ug/106 cells Spectral Red conjugate <= 0.2 ug/106 cells |
| Application Info | Identification and enumeration of CD44+ cells by flow cytometry 1,8, Studies of cell adhesion and homing 4, Immunoprecipitation 1, 4, Western blotting 8 |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution for the product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: 1 Ug/106 cells Cells from BALB/c bone marrow were stained with rat anti-mouse CD44-FITC, following which large cells were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS, pH 7.4, containing 0.1%sodium azide as preservative and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Miyake, K., K. L. Medina, S. Hayashi, S. Ono, T. Hamaoka, and P. W. Kincade. 1990. J. Exp. Med. 171:477 2. Lesley, J., Q. He, K. Miyake, A. Hamann, R. Hyman, and P. W. Kincade. 1992. J. Exp. Med. 175:257 3. Lazaar, A. L. and E. Pure. 1995. The Immunologist 3:19 4. Culty, M., K. Miyake, P. W. Kincade, E. Sikorski, E. C. Butcher, C. Underhill, and E. T. Silorski. 1990. J. Cell Biol. 111:2765 5. Wu, L., P. W. Kincade, and K. Shortman. 1993. Immunol. Lett. 38:69 6. Sommer, F., M. Huber, M. Rollinghoff, and M. Lohoff. 1995. Int. Immunol. 7:1779 7. Murakami, S., K. Miyake, R. Abe, and P. W. Kincade. 1991. J. Immunol. 146:1422 8. He, Q., J. Lesley, R. Hyman, K. Ishihara, and P. W. Kincade. 1992. J. Cell Biol. 119:1711. |
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| Gene Symbol | CD44 |
| Gene Full Name | CD44 antigen |
| Alias Symbols | Pgp, HERM, Ly-2, Ly-24, Pgp-1, HERMES, AU023126, AW121933, AW146109 |
| NCBI Gene Id | 12505 |
| Protein Name | CD44 antigen |
| Uniprot ID | P15379 |
| Protein Accession # | NP_001034240.1 |
| Nucleotide Accession # | NM_001039151.1 |
