Mouse CD90/Thy-1 Antibody : FITC
Référence OASB00535
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Mouse CD90/Thy-1 Antibody : FITC (OASB00535)
| Datasheets/Manuals | Printable datasheet for Mouse CD90/Thy-1 Antibody : FITC (OASB00535) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | G7 |
| Isotype | IgG2c |
| Host | Rat |
| Conjugation | FITC |
| Application | FC, IHC-P |
| Additional Information | Description: CD90 (Thy-1), a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily of cell surface receptors, consists of a single V-set domain. It is expressed on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes, and neurons of all mouse strains. The monoclonal antibody G7 stimulates T-cell proliferation and IL-2 secretion via signaling through the T-cell receptor/CD3 complex. G7 has also been reported to promote apoptosis of thymocytes and CTL clones and to mediate adhesion of thymocytes to thymic stroma. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Mouse T cell hybridoma C6/G8 |
| Concentration | 0.5 mg/mL |
| Specificity | CD90 |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Fluorescein conjugate <= 1 ug/106 cells Biotin conjugate <= 1 ug/106 cells R-phycoerythrin conjugate <= 0.1 ug/106 cells Allophycocyanin conjugate <= 0.2 ug/106 cells Spectral Red conjugate <= 0.2 ug/106 cells |
| Application Info | Flow cytometry 1, In vitro T cell activation 1,5,6,9, Induction of apoptosis 4,7 |
| Other Applications Data | Since applications vary, you should determine the optimum working dilution for the product that is appropriate for yourspecific need. |
| Other Applications Image 1 Data | Amount Used: 1 ug/106 cells BALB/c mesenteric lymph node cells were stained with either rat IgG2c-FITC (as a negative control) or rat anti-mouse CD90-FITC. Lymphocytes were then gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - With the exception of the low endotoxin, azide- free product, the vials should be stored at 2- 8 C Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquotand freeze the low endotoxin, azide- free product at - 20 C immediately upon receipt. Each reagent isstable for the period shown on the bottle label if stored as directed. |
| Reference | 1. Gunter, K., T. Malek, and E. Shevach. 1984. T- cell activating properties of an anti- Thy- 1 monoclonal antibody. J. Exp. Med. 159:716 2. Williams, A.F., and J. Gagnon. 1982. Science 216:696 3. Kroczek, R.A., K.C. Gunter, R.N. Germain, and E.M. Shevach. 1986. Nature 322:181 4. Hueber, A.O., G. Raposo, M. Pierres, and H.T. He. 1994. J. Exp. Med. 179:785 5. Tentori, L., D.M. Pardoll, J.C. Zunigaq, J. Hu- Li, W.E. Paul, J.A. Bluestone, and A.M. Kruisbeek. 1988. J. Immunol. 140:1089 6. Sugiyama, E., A. Cantagrel, T. Reno, F. Stafford- Brady, E.T.H. Yeh, and J.V. Bonventre. 1990. Cell. Immunol. 130:271 7. Ucker, D.S., J. Meyers, and P.S. Obermiller. 1992. J. Immunol. 149:1583 8. He, H- T., P. Naquet, D. Caillol, and M. Pierres. 1991. J. Exp. Med. 173:515 9. Kruisbeek, A.M., and E. Shevach. 1991. Proliferative assays for T cell function. In: Current Protocols in Immunology. Coligan, J., A Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober. John Wiley & Sons, New York, NY, pp. 3.12.1- 3.12.14. |
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| Gene Symbol | THY1 |
| Gene Full Name | Thy-1 membrane glycoprotein |
| Alias Symbols | T25, CD90, Thy-1, Thy1.1, Thy1.2, Thy-1.2 |
| NCBI Gene Id | 21838 |
| Protein Name | thy-1 membrane glycoprotein |
| Description of Target | This gene encodes a glycoprotein that is anchored to the cell surface of thymocytes, neuronal and other cells through a glycosyl-phosphatidylinositol moiety. A soluble form of the encoded protein has also been detected in serum and cerebrospinal fluid. The encoded protein undergoes further processing to generate the mature protein which mediates cell-cell interactions to trigger downstream signaling pathways. |
| Uniprot ID | P01831 |
| Protein Accession # | NP_033408.1 |
| Nucleotide Accession # | NM_009382.3 |
