Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Cr. During the reaction, Mouse Cr in samples or Standard competes with a fixed amount of Mouse Cr on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse Cr. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Mouse Cr in the samples is then determined by comparing the OD of the samples to the standard curve.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse Cr concentrations in serum, plasma and other biological fluids.

Small volumes of Cr elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2504918 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cr, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cr. The ELISA analytical biochemical technique of the MBS2504918 kit is based on Cr antibody-Cr antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cr antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Cr. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).