RayBio® C-Series Mouse Cytokine Antibody Array 1 Kit. Detects 22 Mouse Cytokines. Suitable for all liquid sample types.
Mouse Cytokine Array C1
Mouse Cytokine Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 22 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| GCSF
| GM-CSF
| IL-2
| IL-3
| IL-4
|
| IL-5
| IL-6
| IL-9
| IL-10
| IL-12 p40/p70
|
| IL-12 p70
| IL-13
| IL-17A
| IFN-gamma
| MCP-1 (CCL2)
|
| MCP-5
| RANTES (CCL5)
| SCF
| TNF RI (TNFRSF1A)
| TNF alpha
|
| Thrombopoietin (TPO)
| VEGF-A
| |||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
High baseline tumor burden-associated macrophages promote an immunosuppressive microenvironment and reduce the efficacy of immune checkpoint inhibitors through the IGFBP2-STAT3-PD-L1 pathway
- Species:
- Mouse
- Sample Type:
- MC38 cell-Xenograft, B16 cell-Xenograft
- Year:
- 2023
- Journal Name:
- Cancer Communications
Cellular immune response against nontypeable Haemophilus influenzae infecting the preinflamed middle ear of the Junbo mouse
- Species:
- Mouse
- Sample Type:
- Middle Ear Fluid
- Year:
- 2019
- Journal Name:
- Infection and Immunity
MD-2 is required for disulfide HMGB1-dependent TLR4 signaling
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Mouse Macrophages stimulated with HMGB1)
- Year:
- 2015
- Journal Name:
- Journal of Experimental Medicine
- PubMed ID:
- 25559892
Bidirectional role of IL-6 signal in pathogenesis of lung fibrosis
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Primary alveolar epithelial cells stimulated with bleomycin)
- Year:
- 2015
- Journal Name:
- Respiratory Research
- PubMed ID:
- 26289430
Sex differences in H7N9 influenza A virus pathogenesis
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Lung tissue lysates post H7N9 infection.)
- Year:
- 2015
- Journal Name:
- Vaccine
- PubMed ID:
- 26319064
S100A4-neutralizing antibody suppresses spontaneous tumor progression, pre-metastatic niche formation and alters T-cell polarization balance
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Premetastatic PyV MT mice lungs)
- Year:
- 2015
- Journal Name:
- Bmc Cancer
Glutathione reaction products with a chemical allergen, methylene-diphenyl diisocyanate, stimulate alternative macrophage activation and eosinophilic airway inflammation
- Species:
- Mouse
- Sample Type:
- BAL Fluid (Balb/C treated with gluathione (GSH))
- Year:
- 2015
- Journal Name:
- Chemical Research in Toxicology
- PubMed ID:
- 25635619
A pancreatic tumor-specific biomarker characterized in humans and mice as an immunogenic onco-glycoprotein is efficient in dendritic cell vaccination
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Dendritic Cell supernatues with vaccination based on pancreatic tumors)
- Year:
- 2015
- Journal Name:
- Oncotarget
- PubMed ID:
- 26405163
Yam storage protein dioscorins modulate cytokine gene expression in BALB/c and C57BL/6 lymphocytes
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Spleen, BM, thymus isolated cells treated with PBS or DJ-Dioscorins)
- Year:
- 2015
- Journal Name:
- Food and Agricultural Immunology
Anti-Inflammatory Effects of Water Chestnut Extract on Cytokine Responses via Nuclear Factor-B-signaling Pathway
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Raw264 macrophages)
- Year:
- 2015