Quantibody® Mouse Cytokine Array 1000 Kit. A combination of Mouse Cytokine Array Q4 and Mouse Cytokine Array Q5. Detects 80 Mouse Cytokines. Suitable for all liquid sample types.
Mouse Cytokine Array Q1000
Mouse Cytokine Array Q1000
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 80 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 100 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| Amphiregulin
| Axl
| bFGF
| BLC (CXCL13)
| CD27 Ligand (TNFSF7)
|
| CD30 (TNFRSF8)
| CD30 Ligand (TNFSF8)
| CD40 (TNFRSF5)
| CXCL16
| EGF
|
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| E-Selectin
| Fas Ligand (TNFSF6)
| Fractalkine (CX3CL1)
|
| GCSF
| GITR (TNFRSF18)
| GM-CSF
| HGF
| I-309 (TCA-3/CCL1)
|
| ICAM-1 (CD54)
| IFN-gamma
| IGF-1
| IGFBP-2
| IGFBP-3
|
| IGFBP-5
| IGFBP-6
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 ra (IL-1 F3)
|
| IL-10
| IL-12 p40
| IL-12 p70
| IL-13
| IL-15
|
| IL-17A
| IL-17E (IL-25)
| IL-17F
| IL-2
| IL-2 R alpha
|
| IL-20
| IL-21
| IL-23 p19
| IL-28A (IFN-lambda 2)
| IL-3
|
| IL-4
| IL-5
| IL-6
| IL-7
| I-TAC (CXCL11)
|
| KC (CXCL1)
| Leptin
| LIX
| MCP-1 (CCL2)
| MCP-5
|
| M-CSF
| MDC (CCL22)
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
| MIP-1 gamma
|
| MIP-2
| MIP-3 alpha (CCL20)
| Osteopontin (SPP1)
| Osteoprotegerin (TNFRSF11B)
| Platelet Factor 4 (CXCL4)
|
| Prolactin
| Pro-MMP-9
| P-Selectin
| RANTES (CCL5)
| Resistin
|
| SCF
| SDF-1 alpha (CXCL12 alpha)
| TARC (CCL17)
| Thrombopoietin (TPO)
| TNF alpha
|
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| VCAM-1 (CD106)
| VEGF-A
| VEGF-D
|
Species Detected
Mouse
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Cytokine Array Q1000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAM-CYT-4.gal)
GAL (GAL-QAM-CYT-5.gal)
Excel (QAM-CYT-4_Analysis_Tool.xls)
Excel (QAM-CYT-5_Analysis_Tool.xls)
citations
Cell state dependent effects of Bmal1 on melanoma immunity and tumorigenicity
- Species:
- Mouse
- Sample Type:
- Other / Unspecified (cytokines secreted from YUMM2.1 melanoma cells)
- Year:
- 2024
- Journal Name:
- Nature Communications
- PubMed ID:
- 38245503
Single-Cell Transcriptome Analysis Reveals That Hmga2 Regulates Neuroinflammation and Retinal Function by Modulating Müller Cell Autophagy Through PI3K/AKT Signaling Following MCAO-Induced Retinal Ischemia
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (supernatant from cultured Müller cells)
- Year:
- 2025
- Journal Name:
- Advanced science (Weinheim, Baden-Württemberg, Germany)
- PubMed ID:
- 40884260
Can Urinary Biomarkers at AKI Predict Progression to CKD?
- Species:
- Mouse
- Sample Type:
- Urine (folic acid AKI mice)
- Year:
- 2021
- Journal Name:
- Journal of the American Society of Nephrology
Mutant p53 drives an immune cold tumor immune microenvironment in oral squamous cell carcinoma
- Species:
- Mouse
- Sample Type:
- ROC1 cell
- Year:
- 2022
- Journal Name:
- Communications biology
Identification of in vitro and in vivo oncolytic effect in colorectal cancer cells by Orf virus strain NA1/11
- Species:
- Mouse
- Sample Type:
- Conditioned Media (cell culture media)
- Year:
- 2020
- Journal Name:
- Oncology Reports
Chronic inflammation induces telomere dysfunction and accelerates ageing in mice
- Species:
- Mouse
- Sample Type:
- Conditioned Media (WT vs NFkb-/- mouse ear fibroblasts); Tissue Lysate (Frozen Liver Lysates)
- Year:
- 2014
- Journal Name:
- Nature Communications
Epidermal growth factor regulates hematopoietic regeneration after radiation injury.
- Species:
- Mouse
- Sample Type:
- Bone Marrow Aspirate (BM was collected from adult, non-irradiated Tie2Cre;Bak1?/?;Baxfl/? mice and Tie2Cre;Bak1?/?;Baxfl/+ mice and C57Bl6 mice)
- Year:
- 2013
- Journal Name:
- Nature Medicine
Depletion of tumor-associated macrophages slows the growth of chemically induced mouse lung adenocarcinomas
- Species:
- Mouse
- Sample Type:
- BAL Fluid
- Year:
- 2014
- Journal Name:
- Frontiers in immunology
Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modeling
- Species:
- Mouse
- Sample Type:
- Serum; Tissue Lysate (Liver Lysate)
- Year:
- 2014
- Journal Name:
- Journal of Physiology
- PubMed ID:
- 25384784
Tie2+ bone marrow endothelial cells regulate hematopoietic stem cell regeneration following radiation injury.
- Species:
- Mouse
- Sample Type:
- Bone Marrow Aspirate (mice bearing deletion of the pro-apoptotic proteins, BAK and BAX, Tie2)
- Year:
- 2013
- Journal Name:
- Stem Cells
- PubMed ID:
- 23132593