QAM-CAA-2000-1 | Mouse Cytokine Array Q2000;8 Sample Kit (Glass Slide)

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Mouse Cytokine Array Q2000

Quantibody^® Mouse Cytokine Array 2000 Kit. A combination of Mouse Cytokine Array Q4, Mouse Cytokine Array Q5, and Mouse Cytokine Array Q6. Detects 120 Mouse Cytokines. Suitable for all liquid sample types.

Product Description

Specifications

Size	8 Sample Kit, 22 Sample Kit, 50 Sample Kit
Species	Mouse
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Quantitative
Number of Targets Detected	120
Solid Support	Glass Slide
Design Principle	Sandwich-based
Method Of Detection	Fluorescence Laser Scanner
Shipping Type	Blue ice

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 150 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
4-1BB (TNFRSF9/CD137) Gene Symbol: TNFRSF9 Accession #: P20334 ELISA Kit: RayBio Mouse 4-1BB ELISA	Ace Gene Symbol: ACE Accession #: P22967 ELISA Kit: RayBio Mouse ACE ELISA	ALK-1 Gene Symbol: SLPI Accession #: O09081 ELISA Kit: RayBio Mouse ALK-1 ELISA	Amphiregulin Gene Symbol: AREG Accession #: P31955 ELISA Kit: RayBio Mouse AREG ELISA	Axl Gene Symbol: Axl Accession #: Q00993 ELISA Kit: RayBio Mouse Axl ELISA
bFGF Gene Symbol: FGF2 Accession #: P15655 ELISA Kit: RayBio Mouse bFGF ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Accession #: O55038 ELISA Kit: RayBio Mouse BLC ELISA	Cardiotrophin-1 (CT-1) Gene Symbol: CTF1 Accession #: Q60753 ELISA Kit: RayBio Mouse CT-1 ELISA	CD27 (TNFRSF7) Gene Symbol: CD27 Accession #: P41272 ELISA Kit: RayBio Mouse CD27 ELISA	CD27 Ligand (TNFSF7) Gene Symbol: CD70 Accession #: P32970 ELISA Kit: RayBio Mouse CD27 L ELISA
CD30 (TNFRSF8) Gene Symbol: CD30 Accession #: Q60846 ELISA Kit: RayBio Mouse CD30 ELISA	CD30 Ligand (TNFSF8) Gene Symbol: CD30LG Accession #: P32972 ELISA Kit: RayBio Mouse CD30 L ELISA	CD32a Gene Symbol: FCGR2A Accession #: P08101 ELISA Kit: RayBio Mouse CD32a ELISA	CD40 (TNFRSF5) Gene Symbol: CD40 Accession #: Q99NE2 ELISA Kit: RayBio Mouse CD40 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG Accession #: P27548 ELISA Kit: RayBio Mouse CD40 L ELISA
CTLA-4 (CD152) Gene Symbol: Ctla4 Accession #: Q9QZZ7 ELISA Kit: RayBio Mouse CTLA-4 ELISA	CXCL16 Gene Symbol: CXCL16 Accession #: Q5F2D5 ELISA Kit: RayBio Mouse CXCL16 ELISA	Decorin Gene Symbol: DCN Accession #: P28654 ELISA Kit: RayBio Mouse Decorin ELISA	DKK-1 Gene Symbol: DKK1 Accession #: Q80UL5 ELISA Kit: RayBio Mouse Dkk-1 ELISA	Dtk Gene Symbol: TYRO3 Accession #: O09080 ELISA Kit: RayBio Mouse Dtk ELISA
EGF Gene Symbol: EGF Accession #: E9QNX6 ELISA Kit: RayBio Mouse EGF ELISA	Endoglin (CD105) Gene Symbol: ENG Accession #: Q63961 ELISA Kit: RayBio Mouse Endoglin ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Accession #: P48298 ELISA Kit: RayBio Mouse Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Accession #: Q9JKC0 ELISA Kit: RayBio Mouse Eotaxin-2 ELISA	E-Selectin Gene Symbol: SELE Accession #: Q00690 ELISA Kit: RayBio Mouse E-Selectin ELISA
Fas Ligand (TNFSF6) Gene Symbol: FASLG Accession #: P41047 ELISA Kit: RayBio Mouse Fas L ELISA	Flt-3 Ligand Gene Symbol: FLT3LG Accession #: P49772 ELISA Kit: RayBio Mouse Flt-3L ELISA	Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Accession #: O35188 ELISA Kit: RayBio Mouse Fractalkine ELISA	Galectin-1 Gene Symbol: LGALS1 Accession #: P17601 ELISA Kit: RayBio Mouse Galectin-1 ELISA	Galectin-3 Gene Symbol: LGALS3 Accession #: Q8C253 ELISA Kit: RayBio Mouse Galectin-3 ELISA
Gas 1 Gene Symbol: GAS1 Accession #: Q01721 ELISA Kit: RayBio Mouse GAS1 ELISA	Gas 6 Gene Symbol: Gas6 Accession #: Q99K57 ELISA Kit: RayBio Mouse GAS6 ELISA	GCSF Gene Symbol: CSF3 Accession #: P09920 ELISA Kit: RayBio Mouse G-CSF ELISA	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Accession #: Q9JKR2 ELISA Kit: RayBio Mouse GITR ELISA	GITR Ligand (TNFSF18) Gene Symbol: TNFSF18 Accession #: Q7TS55 ELISA Kit: RayBio Mouse GITR L ELISA
GM-CSF Gene Symbol: CSF2 Accession #: P26955 ELISA Kit: RayBio Mouse GM-CSF ELISA	HAI-1 Gene Symbol: SPINT1 Accession #: Q9R097 ELISA Kit: RayBio Mouse HAI-1 ELISA	HGF Gene Symbol: HGF Accession #: Q53WS5 ELISA Kit: RayBio Mouse HGF ELISA	HGFR Gene Symbol: MET Accession #: D3YVY2 ELISA Kit: RayBio Mouse HGF R ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Accession #: P14098 ELISA Kit: RayBio Mouse TCA-3 ELISA
ICAM-1 (CD54) Gene Symbol: ICAM1 Accession #: P13597 ELISA Kit: RayBio Mouse ICAM-1 ELISA	IFN-gamma Gene Symbol: IFNG Accession #: P01580 ELISA Kit: RayBio Mouse IFN-gamma ELISA	IGF-1 Gene Symbol: IGF1 Accession #: P05018 ELISA Kit: RayBio Mouse IGF-1 ELISA	IGFBP-2 Gene Symbol: IGFBP2 Accession #: P47877 ELISA Kit: RayBio Mouse IGFBP-2 ELISA	IGFBP-3 Gene Symbol: IGFBP3 Accession #: P47878 ELISA Kit: RayBio Mouse IGFBP-3 ELISA
IGFBP-5 Gene Symbol: IGFBP5 Accession #: Q07079 ELISA Kit: RayBio Mouse IGFBP-5 ELISA	IGFBP-6 Gene Symbol: IGFBP6 Accession #: Q91X24 ELISA Kit: RayBio Mouse IGFBP-6 ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Accession #: P01582 ELISA Kit: RayBio Mouse IL-1 alpha ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Accession #: P10749 ELISA Kit: RayBio Mouse IL-1 beta ELISA	IL-1 R4 (ST2) Gene Symbol: IL1RL1 Accession #: Q05208
IL-1 ra (IL-1 F3) Gene Symbol: IL1RN Accession #: P25085 ELISA Kit: RayBio Mouse IL-1 RA ELISA	IL-10 Gene Symbol: IL10 Accession #: Q0VBJ1 ELISA Kit: RayBio Mouse IL-10 ELISA	IL-12 p40 Gene Symbol: IL12B Accession #: P43432	IL-12 p70 Gene Symbol: IL12A Accession #: P43431	IL-13 Gene Symbol: IL13 Accession #: P20109 ELISA Kit: RayBio Mouse IL-13 ELISA
IL-15 Gene Symbol: IL15 Accession #: Q1AHQ7 ELISA Kit: RayBio Mouse IL-15 ELISA	IL-17A Gene Symbol: IL17A Accession #: Q60971 ELISA Kit: RayBio Mouse IL-17A ELISA	IL-17E (IL-25) Gene Symbol: IL25 Accession #: Q8VHH8 ELISA Kit: RayBio Mouse IL-17E ELISA	IL-17F Gene Symbol: IL17F Accession #: Q7TNI7 ELISA Kit: RayBio Mouse IL-17F ELISA	IL-2 Gene Symbol: IL2 Accession #: Q791T3 ELISA Kit: RayBio Mouse IL-2 ELISA
IL-2 R alpha Gene Symbol: IL2RA Accession #: Q61731 ELISA Kit: RayBio Mouse IL-2 R alpha ELISA	IL-20 Gene Symbol: IL20 Accession #: Q9JKV9 ELISA Kit: RayBio Mouse IL-20 ELISA	IL-21 Gene Symbol: IL21 Accession #: E9PX58 ELISA Kit: RayBio Mouse IL-21 ELISA	IL-23 p19 Gene Symbol: IL23A Accession #: Q9EQ14 ELISA Kit: RayBio Mouse IL-23 alpha p19 ELISA	IL-28A (IFN-lambda 2) Gene Symbol: IFNL2 Accession #: Q4VK74 ELISA Kit: RayBio Mouse IL-28 ELISA
IL-3 Gene Symbol: IL3 Accession #: P01586 ELISA Kit: RayBio Mouse IL-3 ELISA	IL-3 R beta Gene Symbol: Csf2rb Accession #: P26955 ELISA Kit: RayBio Mouse IL-3 R beta ELISA	IL-4 Gene Symbol: IL4 Accession #: P07750 ELISA Kit: RayBio Mouse IL-4 ELISA	IL-5 Gene Symbol: IL5 Accession #: P04401 ELISA Kit: RayBio Mouse IL-5 ELISA	IL-6 Gene Symbol: IL6 Accession #: P08505 ELISA Kit: RayBio Mouse IL-6 ELISA
IL-7 Gene Symbol: IL7 Accession #: P10168 ELISA Kit: RayBio Mouse IL-7 ELISA	IL-9 Gene Symbol: IL9 Accession #: P15247 ELISA Kit: RayBio Mouse IL-9 ELISA	I-TAC (CXCL11) Gene Symbol: CXCL11 Accession #: Q9JHH5 ELISA Kit: RayBio Mouse I-TAC ELISA	JAM-A (CD321/F11R) Gene Symbol: F11R Accession #: Q8VC39 ELISA Kit: RayBio Mouse JAM-A ELISA	KC (CXCL1) Gene Symbol: CXCL1 Accession #: P12850 ELISA Kit: RayBio Mouse KC ELISA
Leptin Gene Symbol: LEP Accession #: P41160 ELISA Kit: RayBio Mouse Leptin ELISA	Leptin R Gene Symbol: LEPR Accession #: Q61215 ELISA Kit: RayBio Mouse Leptin R ELISA	LIX Gene Symbol: Cxcl5 Accession #: P50228 ELISA Kit: RayBio Mouse LIX ELISA	L-Selectin (CD62L) Gene Symbol: SELL Accession #: Q3TCF3 ELISA Kit: RayBio Mouse L-Selectin ELISA	Lymphotactin (XCL1) Gene Symbol: XCL1 Accession #: P47993 ELISA Kit: RayBio Mouse Lymphotactin ELISA
MAdCAM-1 Gene Symbol: MADCAM1 Accession #: Q61278 ELISA Kit: RayBio Mouse MAdCAM-1 ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Accession #: Q9QYD7 ELISA Kit: RayBio Mouse MCP-1 ELISA	MCP-5 Gene Symbol: Ccl12 Accession #: Q9QYD6 ELISA Kit: RayBio Mouse MCP-5 ELISA	M-CSF Gene Symbol: CSF1 Accession #: P07141 ELISA Kit: RayBio Mouse M-CSF ELISA	MDC (CCL22) Gene Symbol: CCL22 Accession #: O88430 ELISA Kit: RayBio Mouse MDC ELISA
MFG-E8 Gene Symbol: Mfge8 Accession #: Q9R1X9 ELISA Kit: RayBio Mouse MFG-E8 ELISA	MIG (CXCL9) Gene Symbol: CXCL9 Accession #: P18340 ELISA Kit: RayBio Mouse MIG ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Accession #: P14096 ELISA Kit: RayBio Mouse MIP-1 alpha ELISA	MIP-1 gamma Gene Symbol: Ccl9 Accession #: Q5QNW2 ELISA Kit: RayBio Mouse MIP-1 gamma ELISA	MIP-2 Gene Symbol: Cxcl2 Accession #: P10889 ELISA Kit: RayBio Mouse MIP-2 ELISA
MIP-3 alpha (CCL20) Gene Symbol: CCL20 Accession #: O89093 ELISA Kit: RayBio Mouse MIP-3 alpha ELISA	MIP-3 beta (CCL19) Gene Symbol: CCL19 Accession #: O70460 ELISA Kit: RayBio Mouse MIP-3 beta ELISA	Neprilysin Gene Symbol: MME Accession #: Q6NXX5 ELISA Kit: RayBio Mouse Neprilysin ELISA	Osteopontin (SPP1) Gene Symbol: SPP1 Accession #: P19008 ELISA Kit: RayBio Mouse OPN ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Accession #: O70202 ELISA Kit: RayBio Mouse OPG ELISA
Pentraxin-3 (TSG-14) Gene Symbol: PTX3 Accession #: P48759 ELISA Kit: RayBio Mouse TSG-14 ELISA	Platelet Factor 4 (CXCL4) Gene Symbol: PF4, CXCL4, SCYB4 Accession #: Q9Z126 ELISA Kit: RayBio Mouse PF-4 ELISA	Prolactin Gene Symbol: PRL Accession #: P06879 ELISA Kit: RayBio Mouse Prolactin ELISA	Pro-MMP-9 Gene Symbol: Mmp9 Accession #: Q80XI8 ELISA Kit: RayBio Mouse pro-MMP-9 ELISA	P-Selectin Gene Symbol: SELP Accession #: Q01102 ELISA Kit: RayBio Mouse P-Selectin ELISA
RAGE Gene Symbol: MOK Accession #: Q9WVS4 ELISA Kit: RayBio Mouse RAGE ELISA	RANTES (CCL5) Gene Symbol: CCL5 Accession #: P30882 ELISA Kit: RayBio Mouse RANTES ELISA	Resistin Gene Symbol: RETN Accession #: Q99P87 ELISA Kit: RayBio Mouse Resistin ELISA	SCF Gene Symbol: KITLG Accession #: P20826 ELISA Kit: RayBio Mouse SCF ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Accession #: P40224 ELISA Kit: RayBio Mouse SDF-1 alpha ELISA
TACI (TNFRSF13B) Gene Symbol: TNFRSF13B Accession #: Q9DBZ3 ELISA Kit: RayBio Mouse TACI ELISA	TARC (CCL17) Gene Symbol: CCL17 Accession #: Q9WUZ6 ELISA Kit: RayBio Mouse TARC ELISA	Thrombopoietin (TPO) Gene Symbol: THPO Accession #: P40226 ELISA Kit: RayBio Mouse TPO ELISA	TNF alpha Gene Symbol: TNF Accession #: Q62326 ELISA Kit: RayBio Mouse TNF-alpha ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Accession #: P25118 ELISA Kit: RayBio Mouse sTNFRI ELISA
TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Accession #: O88734 ELISA Kit: RayBio Mouse sTNFRII ELISA	TREM-1 Gene Symbol: TREM1 Accession #: Q3TAL7 ELISA Kit: RayBio Mouse TREM-1 ELISA	TROY (TNFRSF19) Gene Symbol: TNFRSF19 Accession #: Q80T13 ELISA Kit: RayBio Mouse TROY ELISA	TSLP Gene Symbol: TSLP Accession #: Q9JIE6 ELISA Kit: RayBio Mouse TSLP ELISA	TWEAK R (TNFRSF12) Gene Symbol: TNFRSF12A Accession #: Q9CR75 ELISA Kit: RayBio Mouse TWEAK R ELISA
VCAM-1 (CD106) Gene Symbol: VCAM1 Accession #: P29533 ELISA Kit: RayBio Mouse VCAM-1 ELISA	VEGF-A Gene Symbol: VEGFA Accession #: Q00731 ELISA Kit: RayBio Mouse VEGF-A ELISA	VEGF-D Gene Symbol: FIGF Accession #: A2AIH3	VEGFR1 Gene Symbol: Flt1 Accession #: Q61517 ELISA Kit: RayBio Mouse VEGF R1 ELISA	VEGFR3 Gene Symbol: FLT4 Accession #: P35917 ELISA Kit: RayBio Mouse VEGFR-3 ELISA

Species Detected

Mouse

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Mouse Cytokine Array Q2000 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

Datasheet (QAM-CAA-2000.pdf)

SDS (QAM-CAA-2000.pdf)

Excel (QAM-CYT-4_Analysis_Tool.xls)

Excel (QAM-CYT-5_Analysis_Tool.xls)

Excel (QAM-CYT-6_Analysis_Tool.xls)

citations

EphA2 Is a Clinically Relevant Target for Breast Cancer Bone Metastatic Disease
Species:
Mouse
Sample Type:
Cell Culture Supernatant (conditioned medium from 4T1.V, 4T1.ΔC cells)
Year:
2021
Journal Name:
JBMR Plus
PubMed ID:
33869989
Exogenous biological renal support ameliorates renal pathology after ischemia reperfusion injury in elderly mice
Species:
Mouse
Sample Type:
Serum (elderly mice, renal IRI)
Year:
2019
Journal Name:
Aging
PubMed ID:
30978173
Correlates of Vaccine-Induced Protection against Mycobacterium tuberculosis Revealed in Comparative Analyses of Lymphocyte Populations
Species:
Mouse
Sample Type:
Conditioned Media (Co cultures of bone marrow derived macrophates, infected with mycobaterium bovis)
Year:
2015
Journal Name:
Clinical and Vaccine Immunology
PubMed ID:
26269537
Donor Satellite Cell Engraftment is Significantly Augmented When the Host Niche is Preserved and Endogenous Satellite Cells are Incapacitated
Species:
Mouse
Sample Type:
Tissue Lysate (Tibialis anterior muscle lysates)
Year:
2012
Journal Name:
Stem Cells

Mouse Cytokine Array Q2000;8 Sample Kit (Glass Slide)

Référence QAM-CAA-2000-1

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