Mouse IgG2a Kappa isotype Control Antibody

Référence orb343763-1mg

Conditionnement : 1mg

Marque : Biorbyt

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Mouse IgG2a Kappa isotype Control Antibody

SKU: orb343763

Description

Mouse IgG2a Kappa isotype Control Antibody

Research Area

Protein Biochemistry

Images & Validation

Tested ApplicationsELISA, FC, SDS-PAGE, WB
Dilution RangeFC: 1:1000-1:5000
Application Notes
Mouse IgG2a kappa isotype control has been tested in SDS-Page, ELISA, and western blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Key Properties

IsotypeIgG2a
Clone No.M2AK
PurityMouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Concentration1.009 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Mouse IgG2a isotype control, Mouse IgG2a subclass isotype control, Mouse IgG2a Kappa

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

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Référence
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Prix HT
1155-09L
 0.2mg