Mouse NK1.1 Antibody : LE/AF
Référence OASB00611
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Mouse NK1.1 Antibody : LE/AF (OASB00611)
| Datasheets/Manuals | Printable datasheet for Mouse NK1.1 Antibody : LE/AF (OASB00611) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | PK136 |
| Isotype | IgG2a |
| Host | Mouse |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC, IP, CMCD, BL |
| Additional Information | Description: NK1.1, a member of the NKR-P1 family of cell surface receptors, is a type II integral membrane glycoprotein with a C-type lectin domain. It is expressed as a disulfide-linked homodimer on all NK cells as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.g., C57BL/6, NZB, and CE). NK1.1 mediates cellular activation and differentiation, and is thought to have a particular role in generating Th2 cells. This product does not react with NK cells of BALB/c mice. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | CE mouse spleen enriched for NK-1+ cells and bone marrow cells |
| Concentration | 0.5 mg/mL |
| Specificity | NK1.1 |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conformto characteristics of a standard reference reagent. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate R-phycoerythrin-Cy 7 conjugate Allophycocyanin conjugate <=1 ug/106 cells <=1 ug/106 cells <=0.5 ug/106 cells <= 0.3 ug/106 cells <=0.5 ug/106 cells |
| Application Info | Flow cytometry 3, 10, 13, Induction of proliferation of a subset of NK cells 11, Immunoprecipitation 9, In vivo depletion of NK1.1 cells 3, 5, 14, In vitro redirected lysis 12, In vitro cytotoxicity assays 13, 14 |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: 0.5 ug/106 cells Splenocytes from a C57BL/6 mouse were double stained with mouse anti-mouse NK1.1-PE and rat anti-mouse CD49b/pan NK-FITC (clone DX5). Small lymphocytes were then gated and analyzed by flow cytometry. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The R- phycoerythrin- Cyanine 7 (R- PE- CY 7) conjugates are supplied as 0.1 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Michie. S.A., P.R. Streeter, E.C. Butcher, and R.V. Rouse. 1995. Am. J. Pathol. 147:412 2. Garni- Wagner, B.A., A. Purohit, P.A. Matthew, M. Bennett, and V. Kumar. 1993. J. Immunol. 151:60 3. Koo, G.C., F.J. Dumont, M. Tutt, J. Hackett, Jr., and V. Kumar. 1986. J. Immunol. 137:3742 4. Yokoyama, W.M., and W.E. Seaman. 1993. Annu, Rev. Immunol. 11:613 5. Sentman, C.L., V. Kumar, G. Koo, and M. Bennett. 1989. J. Immunol. 142:1847 6. Yu, Y.Y.L., V. Kumar, and M. Bennett. 1992. Annu. Rev. Immuonol. 10:189 7. Vicari, A.P., and A. Zlotnik. 1996. Immunol. Today 17:71 8. Lanier, L.L. 1997. Immunity 6:371 9. Sentman, C.L., J. Hackett, Jr., T.A. Moore, M.M. Tutt, M. Bennett, and V. Kumar. 1989. Hybridoma 8:605 10. Koo, G., and J. Peppard. 1984. Hybridoma 3:301 11. Reichlin, A., and W.M. Yokoyama. 1998. Immunol. Cell Biol. 76:143 12. Karlhofer, F.M., and W.M. Yokoyama. 1991. J. Immunol. 146:3662 13. Poulton, L.D., M.J. Smyth, C.G. Hawke, P. Silveria, D. Shepherd, O.V. Naidenko, D.I. Godfrey, and A.G. Baxter. 2001. Int. Immunol. 13:887 14. Routes, J.M., J.C. Ryan, S. Ryan, and M. Nakamura. 2001. Int. Immunol. 13:1301. |
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| Gene Symbol | KLRB1C |
| Gene Full Name | Killer cell lectin-like receptor subfamily B member 1C |
| Alias Symbols | Nk, Ly5, NK-, NKR, Nk1, Ly-5, Ly55, Ly59, NKR-, Nk-1, Nk1., Nkrp, CD161, Ly-59, Ly55c, NK-1., NK1.1, NKRP1, Nk1.2, Klrb1b, NK-RP1, Nk-1.2, Nkrp1b, Nkrp1c, ly-55c, NKRP140, AI462337, NKR-P1.9 |
| NCBI Gene Id | 17059 |
| Protein Name | killer cell lectin-like receptor subfamily B member 1C |
| Uniprot ID | P27814 |
| Protein Accession # | NP_001153376.1 |
| Nucleotide Accession # | NM_001159904.1 |
