Mouse Nos3 (Nitric oxide synthase, endothelial) Sandwich ELISA E0004531)

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	Nos3
Gene ID:	18127
Uniprot ID:	NOS3_MOUSE
Specificity:	This kit recognizes Mouse NOS3/eNOS in samples. No significant cross-reactivity or interference between Mouse NOS3/eNOS and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.
Protein Name	Nitric Oxide Synthase 3 Constitutive Nos Cnos Ec-Nos Nos Type Iii Nosiii Nitric Oxide Synthase - Endothelial Endothelial Nos Enos
Database Links	Reactome: R-MMU-1222556 Reactome: -MMU-1474151 Reactome: -MMU-203615 Reactome: -MMU-203641 Reactome: -MMU-203754 Reactome: -MMU-392154 Reactome: -MMU-5218920 Reactome: -MMU-9009391
Cellular Localisation	Membrane Caveola Cytoplasm Cytoskeleton Golgi Apparatus Cell Membrane Specifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell Cycle Which Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity
Alternative ELISA Names	Nitric Oxide Synthase 3 ELISA kit Constitutive Nos ELISA kit Cnos ELISA kit Ec-Nos ELISA kit Nos Type Iii ELISA kit Nosiii ELISA kit Nitric Oxide Synthase - Endothelial ELISA kit Endothelial Nos ELISA kit Enos ELISA kit Nos3 ELISA kit Ecnos ELISA kit
Specificity	This kit recognizes Mouse NOS3/eNOS in samples. No significant cross-reactivity or interference between Mouse NOS3/eNOS and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-108	99
EDTA plasma (n=8)	90-104	97
Cell culture media (n=8)	92-104	99

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	45.50	109.20	426.30	44.30	119.40	394.80
Standard deviation	3.20	5.10	17.90	2.40	6.20	18.60
CV (%)	7.03	4.67	4.20	5.42	5.19	4.71

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance