MTT-Cell Based Proliferation/Toxicity Assay Kit

Background: Measurement of cell viability and proliferation forms the basis for numerous in vitro assays of a cell population's response to external factors. The reduction of tetrazolium salts is now widely accepted as a reliable way to examine cell proliferation. The key substrate of the kit is:3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide or MTT. MTT is a yellowish solution when dissolved in balanced salt solutions without phenol red and is taken up by cells due to its net positive charge. The tetrazolium ring of MTT (yellow) is reduced to purple formazan crystals by intracellular NAD(P)H-oxidoreductases. The formazan crystals are insoluble in aqueous solution, but become solubilized in the provided solubilization Buffer. After solubilization, the resulting purple solution is spectrophotometrically measured. The MTT Cell Proliferation Assay measures the cell proliferation rate and conversely, when metabolic events lead to apoptosis or necrosis, the reduction in cell viability. The number of assay steps has been minimized as much as possible to expedite sample processing. The MTT solution yields low background absorbance values in the absence of cells. For each cell type the linear relationship between cell number and signal produced is established, thus allowing an accurate quantification of changes in the rate of cell proliferation.

Small volumes of MTT assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7608272 is an assay kit targeting MTT. Also known as MTT-Cell Based Proliferation/Toxicity Assay Kit.