Nod1 (NM_172729) Mouse Tagged ORF Clone
CAT#: MG225900
- TrueORF®
Nod1 (tGFP-tagged) - Mouse nucleotide-binding oligomerization domain containing 1 (Nod1) transcript variant 1, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
Product Images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | Nod1 |
| Synonyms | C230079P11; Card4; F830007N14Rik; Nlrc1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >MG225900 representing NM_172729 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAACACGGCCATCATGAGATGGAAGGCACCCCATTGGGTTGTCACTCCCACATTAAACTGCTGA AGATCAACAGGGAACATCTGGTCACCAACATTCGGAACACTCAGTGTCTGGTGGACAACTTGCTGGAGAA TGGCTACTTCTCAGCCGAAGATGCAGAGATTGTGTGTGCCTGTCCCACCAAGCCTGACAAGGTCCGAAAG ATCCTTGACCTGGTGCAGAGCAAAGGCGAGGAGGTGTCTGAGTTCTTCCTCTACGTGCTGCAGCAGCTGG AGGATGCTTACGTGGACCTCAGGCTGTGGCTCTCAGAAATTGGCTTCTCCCCTTCCCAGCTCATTCGGAC CAAAACTATCGTCAATACTGACCCAGTAAGCAGGTATACCCAACAGCTGCGACACCAACTGGGCCGCGAC TCCAAGTTCATGCTGTGCTACGCCCAGAAGGAGGACCTGCTGCTGGAGGAGACCTATATGGACACACTCA TGGAGCTGGTAGGCTTCAACAATGAAAACCTGGGCAGCCTAGGAGGCCTGGATTGCCTGCTGGACCACAG TACGGGCGTCCTCAACGAGCATGGCGAGACTGTCTTCGTGTTCGGGGACGCGGGAGTGGGCAAGTCCATG CTGCTGCAGAGGTTGCAGAGCCTCTGGGCGTCAGGCAGGTTGACCTCCACAGCCAAATTCTTCTTCCACT TCCGCTGCCGCATGTTCAGCTGCTTCAAGGAGAGCGACATGCTGAGTCTGCAGGACCTGCTCTTCAAGCA TTTCTGCTACCCGGAGCAGGACCCCGAGGAGGTGTTCTCCTTCTTGCTGCGCTTTCCCCACACAGCGCTC TTCACTTTTGACGGCCTGGATGAGCTGCACTCAGACTTCGACCTGAGCCGCGTGCCGGATAGCTGCTGCC CCTGGGAGCCGGCTCACCCTCTGGTCCTGCTGGCTAACCTCCTAAGTGGGAGGCTGCTCAAGGGTGCCGG CAAATTGCTCACTGCTCGCACAGGCGTGGAGGTCCCCCGCCAGCTCCTGCGCAAAAAGGTGCTGCTCCGG GGCTTCTCCCCAAGTCACCTGCGCGCCTATGCCCGCCGGATGTTCCCCGAGCGCACAGCGCAGGAGCATC TGCTGCAGCAGCTGGATGCCAACCCCAACCTCTGCAGCCTGTGCGGGGTGCCGCTCTTCTGTTGGATCAT CTTCCGTTGTTTCCAGCACTTCCAGACGGTCTTCGAGGGCTCCTCTTCACAGTTGCCGGACTGTGCTGTG ACCCTGACCGATGTCTTTCTGCTGGTCACTGAGGTGCATCTGAACAGGCCGCAGCCCAGCAGCCTGGTGC AGCGCAACACGCGCAGCCCGGCGGAAACCCTACGTGCAGGCTGGCGCACGCTGCATGCGCTGGGAGAGGT GGCTCACCGAGGCACCGACAAGAGCCTCTTTGTGTTTGGCCAGGAGGAGGTGCAGGCGTCGAAGCTGCAG GAAGGAGATCTGCAGCTGGGCTTCCTGCGGGCTTTGCCCGATGTGGGCCCTGAGCAGGGCCAGTCTTACG AATTTTTCCACCTTACGCTCCAGGCCTTCTTCACCGCCTTCTTCCTGGTAGCAGATGACAAAGTGAGCAC CCGGGAGTTGCTGAGGTTCTTTCGAGAATGGACGTCTCCTGGAGAGGCAACAAGCTCGTCCTGCCATTCT TCCTTCTTCTCCTTCCAGTGCCTGGGCGGCAGAAGCCGGTTGGGCCCTGATCCTTTCAGGAACAAAGATC ACTTCCAGTTCACCAACCTCTTCCTGTGCGGGCTACTGGCCAAAGCCCGACAGAAACTCCTTCGGCAGCT GGTGCCCAAGGCTATCCTGAGGAGGAAGCGCAAGGCCCTGTGGGCTCACCTGTTTGCTAGCCTGCGCTCC TACTTGAAGAGCCTACCTCGGGTCCAGTCTGGAGGCTTTAACCAGGTGCATGCCATGCCCACATTCCTGT GGATGCTGCGCTGCATCTATGAGACGCAGAGCCAGAAGGTGGGGCGCCTCGCCGCCAGGGGCATCAGTGC GGACTACCTCAAGCTGGCCTTTTGCAACGCTTGCTCTGCGGACTGCAGCGCCCTGTCCTTCGTCCTGCAT CACTTCCACAGGCAGCTGGCCCTAGACCTGGACAACAACAACCTCAATGACTATGGCGTGCAGGAGCTGC AGCCTTGCTTTAGCCGTCTCACGGTTATCAGACTCAGCGTCAACCAGATCACCGACACGGGGGTGAAGGT GCTATGTGAGGAACTGACCAAGTATAAGATCGTGACGTTCCTGGGTTTATACAACAACCAGATAACTGAT ATCGGAGCCAGGTATGTGGCCCAAATCCTGGATGAATGCAGAGGCCTCAAGCACCTTAAACTAGGGAAAA ACAGAATAACAAGTGAGGGCGGGAAGTGTGTGGCTTTGGCTGTGAAGAACAGCACCTCCATCGTTGATGT TGGGATGTGGGGTAATCAGATTGGAGACGAAGGGGCAAAGGCCTTCGCAGAGGCATTGAAGGACCACCCC AGCCTGACCACTCTCAGTCTTGCATTCAATGGCATCTCTCCGGAGGGAGGGAAGAGCCTTGCGCAGGCCC TGAAGCAGAACACCACACTGACAGTAATCTGGCTGACCAAAAATGAACTTAATGATGAGTCTGCAGAGTG CTTCGCTGAGATGCTGAGAGTGAACCAGACGCTACGGCATTTATGGCTGATCCAGAATCGCATCACAGCC AAGGGGACAGCGCAGCTGGCGAGGGCACTGCAGAAGAACACAGCCATAACAGAGATTTGTCTCAATGGAA ACTTGATTAAGCCCGAGGAGGCCAAAGTCTTCGAGAATGAGAAGAGAATCATCTGCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225900 representing NM_172729 Red=Cloning site Green=Tags(s) MEEHGHHEMEGTPLGCHSHIKLLKINREHLVTNIRNTQCLVDNLLENGYFSAEDAEIVCACPTKPDKVRK ILDLVQSKGEEVSEFFLYVLQQLEDAYVDLRLWLSEIGFSPSQLIRTKTIVNTDPVSRYTQQLRHQLGRD SKFMLCYAQKEDLLLEETYMDTLMELVGFNNENLGSLGGLDCLLDHSTGVLNEHGETVFVFGDAGVGKSM LLQRLQSLWASGRLTSTAKFFFHFRCRMFSCFKESDMLSLQDLLFKHFCYPEQDPEEVFSFLLRFPHTAL FTFDGLDELHSDFDLSRVPDSCCPWEPAHPLVLLANLLSGRLLKGAGKLLTARTGVEVPRQLLRKKVLLR GFSPSHLRAYARRMFPERTAQEHLLQQLDANPNLCSLCGVPLFCWIIFRCFQHFQTVFEGSSSQLPDCAV TLTDVFLLVTEVHLNRPQPSSLVQRNTRSPAETLRAGWRTLHALGEVAHRGTDKSLFVFGQEEVQASKLQ EGDLQLGFLRALPDVGPEQGQSYEFFHLTLQAFFTAFFLVADDKVSTRELLRFFREWTSPGEATSSSCHS SFFSFQCLGGRSRLGPDPFRNKDHFQFTNLFLCGLLAKARQKLLRQLVPKAILRRKRKALWAHLFASLRS YLKSLPRVQSGGFNQVHAMPTFLWMLRCIYETQSQKVGRLAARGISADYLKLAFCNACSADCSALSFVLH HFHRQLALDLDNNNLNDYGVQELQPCFSRLTVIRLSVNQITDTGVKVLCEELTKYKIVTFLGLYNNQITD IGARYVAQILDECRGLKHLKLGKNRITSEGGKCVALAVKNSTSIVDVGMWGNQIGDEGAKAFAEALKDHP SLTTLSLAFNGISPEGGKSLAQALKQNTTLTVIWLTKNELNDESAECFAEMLRVNQTLRHLWLIQNRITA KGTAQLARALQKNTAITEICLNGNLIKPEEAKVFENEKRIICF TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_172729 |
| ORF Size | 2859 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172729.3, NP_766317.1 |
| RefSeq Size | 4327 bp |
| RefSeq ORF | 2862 bp |
| Locus ID | 107607 |
| UniProt ID | Q8BHB0 |
| Cytogenetics | 6 B3 |
| Gene Summary | Enhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Recruits NLRP10 to the cell membrane following bacterial infection (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| MC205444 | Nod1 (untagged) - Mouse nucleotide-binding oligomerization domain containing 1 (Nod1), transcript variant 1, (10ug) | 873,00 EUR | |
| MG211295 | Nod1 (tGFP-tagged) - Mouse nucleotide-binding oligomerization domain containing 1 (Nod1) | 1 510,00 EUR | |
| MR225900 | Nod1 (Myc-DDK-tagged) - Mouse nucleotide-binding oligomerization domain containing 1 (Nod1), transcript variant 1 | 873,00 EUR | |
| MR225900L3 | Lenti ORF clone of Nod1 (Myc-DDK-tagged) - Mouse nucleotide-binding oligomerization domain containing 1 (Nod1), transcript variant 1 | 1 173,00 EUR | |
| MR225900L4 | Lenti ORF clone of Nod1 (mGFP-tagged) - Mouse nucleotide-binding oligomerization domain containing 1 (Nod1), transcript variant 1 | 1 173,00 EUR |