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> Octaethylene glycol monododecyl ether [3055-98-9]

Octaethylene glycol monododecyl ether [3055-98-9]

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Référence HY-138941-1mL

Conditionnement : 10mM/1mL

Marque : MedChemExpress

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Description

Octaethylene glycol monododecyl ether (C12E8) is an non-ionic detergent that can be used for membrane protein extraction. Octaethylene glycol monododecyl ether can solubilize the viral membrane of intact influenza virus[1][2].

Cellular Effect
Cell Line Type Value Description References
MOLT-4 IC50
29 μM
Compound: HL-8
Growth inhibition of HIV1 3B infected human MOLT4 cells after 5 days by MTT assay
Growth inhibition of HIV1 3B infected human MOLT4 cells after 5 days by MTT assay
[PMID: 18672367]
MOLT-4 IC50
59 μM
Compound: HL-8
Growth inhibition of human MOLT4 cells after 5 days by MTT assay
Growth inhibition of human MOLT4 cells after 5 days by MTT assay
[PMID: 18672367]
In Vitro

Octaethylene glycol monododecyl ether (C12E8) has been shown to solubilize the viral membrane of intact influenza virus. The mechanism of solubilization of the viral membrane is based on the accumulation of C12E8 molecules outside the membrane until the formation of micelles leading to the extraction of membrane constituents[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Octaethylene glycol monododecyl ether Related Antibodies

Solvant et solubilité
In Vitro: 

H2O : ≥ 100 mg/mL (185.61 mM)

DMSO : 100 mg/mL (185.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8561 mL 9.2807 mL 18.5615 mL
5 mM 0.3712 mL 1.8561 mL 3.7123 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation
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