Prolactin Rabbit Protein
Référence OPPA00772
Conditionnement : 10µg
Marque : Aviva Systems Biology
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Prolactin Rabbit Protein (OPPA00772)
| Datasheets/Manuals | Printable datasheet for Prolactin Rabbit Protein (OPPA00772) |
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| Predicted Species Reactivity | Rabbit |
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| Product Format | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
| Host | E. Coli |
| Additional Information | Solubility: It is recommended to reconstitute the lyophilized Prolactin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
| :: | Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of rat lymphoma, Nb2-11 was found to be < 0.065 ng/ml. |
| :: | Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.87 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Prolactinl as a Reference Standard. Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.87 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Prolactinl as a Reference Standard. |
| :: | Product Introduction: Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation. Product Description: Prolactin Rabbit Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 23007 Dalton. The Porlactin is purified by proprietary chromatographic techniques. |
| Reconstitution and Storage | Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Prl should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity | Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Peptide Sequence | The sequence of the first five N - terminal amino acids was determined and was found to be Ala - Leu - Pro - Ile - Cys. |
| Gene Symbol | raProlactin |
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| Alias Symbols | GHA1 |
| NCBI Gene Id | 100009394 |
| Protein Name | Prolactin |
| Description of Target | Recombinant Rabbit Prolactin |
| Uniprot ID | Q28632 |
| Protein Accession # | NP_001076144.1 |
| Protein Size (# AA) | Recombinant |