Rabbit Anti-TSPAN6 (C-term) Polyclonal Antibody

Référence AP9224b

Conditionnement : 400ul

Marque : Abcepta

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TSPAN6 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

 
  • WB - TSPAN6 Antibody (C-term) AP9224b

    Western blot analysis of TSPAN6 Antibody (C-term) (Cat. #AP9224b) in mouse bladder tissue lysates (35ug/lane). TSPAN6 (arrow) was detected using the purified Pab.

  • WB - TSPAN6 Antibody (C-term) AP9224b

    Western blot analysis of TSPAN6 (arrow) using rabbit polyclonal TSPAN6 Antibody (C-term) (Cat. #AP9224b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TSPAN6 gene.

  • FC - TSPAN6 Antibody (C-term) AP9224b

    TSPAN6 Antibody (C-term) (Cat. #AP9224b) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of TSPAN6 Antibody (C-term) (Cat. #AP9224b) in mouse bladder tissue lysates (35ug/lane). TSPAN6 (arrow) was detected using the purified Pab.

Product Information

Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession O43657
Reactivity Human, Mouse
Predicted Rat, Bovine, Canine, Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 27563 Da
Antigen Region 176-205 aa

Additional Information

Gene ID 7105
Other Names Tetraspanin-6, Tspan-6, A15 homolog, Putative NF-kappa-B-activating protein 321, T245 protein, Tetraspanin TM4-D, Transmembrane 4 superfamily member 6, TSPAN6, TM4SF6
Target/Specificity This TSPAN6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176~205 amino acids from the C-terminal region of human TSPAN6.
Dilution WB~~1:1000
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTSPAN6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information

Name TSPAN6
Synonyms TM4SF6
Cellular Location Membrane; Multi-pass membrane protein.
Research Areas
  • Primary Antibodies
  • Signal Transduction
Citations ( 4 )
Tspan6 stimulates the chemoattractive potential of breast cancer cells for B cells in an EV- and LXR-dependent manner
Author : Guerman Molostvov 1, Mariam Gachechiladze 2, Abeer M Shaaban 1, Steven Hayward 1, Isaac Dean 3, Irundika H K Dias 4, Nahla Badr 5, Irini Danial 1, Fiyaz Mohammed 3, 
Cell Rep .2023 Mar 28;42(3):112207. doi: 10.1016/j.celrep.2023.112207. Epub 2023 Mar 3.
Application:
36867531
Click Chemistry-mediated Biotinylation Reveals a Function for the Protease BACE1 in Modulating the Neuronal Surface Glycoproteome.
Author : Herber J1,2,Njavro J1,2,Feederle R1,3,4,Schepers U5,Müller UC6,Bräse S5,Müller SA1,2,Lichtenthaler SF7,2,3,8.
Mol Cell Proteomics. 2018 Aug;17(8):1487-1501. doi: 10.1074/mcp.RA118.000608. Epub 2018 May 1.
Application: WB
29716987
Tetraspanin 6: a pivotal protein of the multiple vesicular body determining exosome release and lysosomal degradation of amyloid precursor protein fragments.
Author : Guix FX1,2,Sannerud R3,4,Berditchevski F5,Arranz AM3,4,Horré K3,4,Snellinx A3,4,Thathiah A6,Saido T7,Saito T7,Rajesh S5,Overduin M8,Kumar-Singh S9,Radaelli E3,4,Corthout N3,4,Colombelli J10,Tosi S10,Munck S3,4,Salas IH3,4,Annaert W3,4,De Strooper B11,12,1
Mol Neurodegener. 2017 Mar 10;12(1):25. doi: 10.1186/s13024-017-0165-0.
Application: WB
28279219
Tetraspanin 6: A novel regulator of hippocampal synaptic transmission and long term plasticity.
Author : Salas IH1,2,Callaerts-Vegh Z3,Arranz AM1,2,Guix FX1,2,D'Hooge R3,Esteban JA4,De Strooper B1,2,Dotti CG4.
PLoS One. 2017 Feb 16;12(2):e0171968. doi: 10.1371/journal.pone.0171968. eCollection 2017.
Application: WB
28207852
Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol
Blocking Peptides Protocol
Dot Blot Protocol
Immunohistochemistry Protocol
Immunofluorescence Protocol
Immunoprecipitation Protocol
Flow Cytomety Protocol
Cell Culture Protocol

Background

The protein is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein and is highly similar in sequence to the transmembrane 4 superfamily member 2.

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Référence
Description
Cond.
Prix HT
102-27684
 400uL