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> Rabbit IL-6 ELISA Kit

Rabbit IL-6 ELISA Kit

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Référence ELL-IL6-2

Conditionnement : 2PlateKit

Marque : RayBiotech

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Téléphone : +1 850 650 7790

25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

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Rabbit IL-6 ELISA Kit

Rabbit IL-6 ELISA Kit

RayBio® Rabbit IL-6 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesRabbit
Sensitivity
0.4 pg/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
0.4 pg/ml - 100 pg/ml
Specificity
This ELISA antibody pair detects Rabbit IL-6. Other species not determined.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
2 - 10 fold
Accession Number
Q9MZR1
Gene Id
100008733
Gene Symbols
IL6
Protein Name / Synonyms
Interleukin-6 (IL-6)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaCardiovascular Disease, Inflammation
Shipping TypeBlue ice
Storage-20°C

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Rabbit IL-6 ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified IL-6 (IL6), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELL-IL6.pdf)
SDS (ELL-IL6.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Modulation of the Acute Inflammatory Response Induced by the Escherichia coli Lipopolysaccharide through the Interaction of Pentoxifylline and Florfenicol in a Rabbit Model

    Species:
    Rabbit
    Sample Type:
    Plasma
    Year:
    2023
    Journal Name:
    Antibiotics-Basel

  • Low-Molecular-Weight Collagen Peptide Ameliorates Osteoarthritis Progression through Promoting Extracellular Matrix Synthesis by Chondrocytes in a Rabbit Anterior Cruciate Ligament Transection Model

    Species:
    Rabbit
    Sample Type:
    Other Body Fluid (synovial fluid)
    Year:
    2021
    Journal Name:
    Journal of microbiology and biotechnology
    PubMed ID:
    34528913

  • Physiologically variable ventilation in a rabbit model of asthma exacerbation

    Species:
    Rabbit
    Year:
    2020

  • S-Methylcysteine (SMC) Ameliorates Intestinal, Hepatic, and Splenic Damage Induced by Cryptosporidium parvum Infection Via Targeting Inflammatory Modulators and Oxidative Stress in Swiss Albino Mice

    Species:
    Rabbit
    Sample Type:
    Serum
    Year:
    2020
    Journal Name:
    Biomedicines

  • Benefit of Physiologically Variable Over Pressure-Controlled Ventilation in a Model of Chronic Obstructive Pulmonary Disease: A Randomized Study

    Species:
    Rabbit
    Sample Type:
    Bronchoalveolar Lavage (supernatant of the BALF); Tissue Lysate / Homogenate (lung tissue homogenates)
    Year:
    2020
    Journal Name:
    Frontiers in Physiology
    PubMed ID:
    33519528

  • NCX 470 Exerts Retinal Cell Protection and Enhances Ophthalmic Artery Blood Flow After Ischemia/Reperfusion Injury of Optic Nerve Head and Retina

    Species:
    Rabbit
    Sample Type:
    retina
    Year:
    2023
    Journal Name:
    Translational Vision Science & Technology