Rat Anti-Mouse Lambda-AP
Référence MBS673052-1mL
Conditionnement : 1mL
Marque : MyBiosource
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Product: Rat Anti-Mouse lambda-R-PE
Amount Used: < or = 0.2 ug/10^6 cells
BALB/c splenocytes were double-stained with rat anti-mouse lambda-R-PE and rat anti-mouse C019-FITC (Clone 6D5). Small lymphocytes were then gated and analyzed using a flow cytometer.
Amount Used: < or = 0.2 ug/10^6 cells
BALB/c splenocytes were double-stained with rat anti-mouse lambda-R-PE and rat anti-mouse C019-FITC (Clone 6D5). Small lymphocytes were then gated and analyzed using a flow cytometer.
Rat Anti-Mouse Lambda (Lambda Light Chain Specific) Secondary Antibody – Rat Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673052MBS673052 is a secondary antibody targeting Rat Anti-Mouse Lambda (lambda light chain specific). Also known as Rat Anti-Mouse Lambda-AP. Reactive in Mouse. Host: Rat. More details
Product Overview
Online Datasheet in PDFProduct Name
Rat Anti-Mouse Lambda (lambda light chain specific), Secondary Antibody
Full Product Name
Rat Anti-Mouse Lambda-AP
Product Synonym Names
Rat Anti-Mouse Lambda- Alkaline Phosphatase-Labeled; Rat Anti-Mouse Lambda (lambda light chain specific)
Clonality
Monoclonal
Isotype
Rat IgG2b kappa
Clone Number
JC5-1
Host
Rat
Species Reactivity
Mouse
Form/Format
Alkaline Phosphatase (AP) Conjugate
Specificity
Reacts with mouse lambda light chains as demonstrated by ELISA, FLISA, and/or flow cytometry.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry and/or ELISA to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Product Categories/Family for Rat Anti-Mouse Lambda (lambda light chain specific) secondary antibody
Rat Anti-Mouse Lambda
Applications Tested/Suitable for Rat Anti-Mouse Lambda (lambda light chain specific) secondary antibody
ELISA; Flow Cytometry; Immunofluorescence
Application Notes for Rat Anti-Mouse Lambda (lambda light chain specific) secondary antibody
Research Application(s): Identification and enumeration of lambda ^+ cells by flow cytometry
Identification and enumeration of lambda ^+ cells by immunofluorescence microscopy
ELISA:
Alkaline phosphatase conjugate: 1:1,000-1:4,000
Identification and enumeration of lambda ^+ cells by immunofluorescence microscopy
ELISA:
Alkaline phosphatase conjugate: 1:1,000-1:4,000
IMMUNOFLUORESCENT STAINING of Rat Anti-Mouse Lambda (lambda light chain specific) secondary antibody
Product: Rat Anti-Mouse lambda-R-PE
Amount Used: < or = 0.2 ug/10^6 cells
BALB/c splenocytes were double-stained with rat anti-mouse lambda-R-PE and rat anti-mouse C019-FITC (Clone 6D5). Small lymphocytes were then gated and analyzed using a flow cytometer.
Amount Used: < or = 0.2 ug/10^6 cells
BALB/c splenocytes were double-stained with rat anti-mouse lambda-R-PE and rat anti-mouse C019-FITC (Clone 6D5). Small lymphocytes were then gated and analyzed using a flow cytometer.
Other Notes
Small volumes of Rat Anti-Mouse Lambda (lambda light chain specific) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.