Rat Anti-Mouse Ly-6A/E-PE
Référence MBS673481-0,2mg
Conditionnement : 0.2mg
Marque : MyBiosource
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Ly-6a/E (Sca-1) Secondary Antibody – Rat Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673481MBS673481 is a secondary antibody targeting Ly-6A/E (Sca-1). Also known as Rat Anti-Mouse Ly-6A/E-PE. Reactive in Mouse. Host: Rat. More details
Product Overview
Online Datasheet in PDFProduct Name
Ly-6A/E (Sca-1), Secondary Antibody
Full Product Name
Rat Anti-Mouse Ly-6A/E-PE
Product Synonym Names
Rat Anti-Mouse Ly-6A/E - Phycoerythrin-Labeled; Rat Anti-Mouse Ly-6A/E (Sca-1)
Clonality
Monoclonal
Isotype
Rat (Lewis) IgG2a kappa
Clone Number
D7
Host
Rat
Species Reactivity
Mouse
Form/Format
R - phycoerythrin (R - PE) Conjugate
Specificity
Ly-6A.2 and Ly-6E.1, allelic members of the Ly-6 multigene family; Mr 18 kDa
Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins.^1-3 It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes.^4-6 In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E^+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E^+ lymphocytes.^1, 7-9 The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.^1, 8, 9 Studies with MAb D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses,^9-12 and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.^3, 13
Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins.^1-3 It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes.^4-6 In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E^+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E^+ lymphocytes.^1, 7-9 The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.^1, 8, 9 Studies with MAb D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses,^9-12 and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.^3, 13
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Immunogen
IL-2-dependent mouse T-cell line CTL-L
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative flow data are included in this product insert.
Applications Tested/Suitable for Ly-6A/E (Sca-1) secondary antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot
Application Notes for Ly-6A/E (Sca-1) secondary antibody
Research Application(s): In vitro lymphocyte activation
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Product References and Citations for Ly-6A/E (Sca-1) secondary antibody
1. Ortega, G., P.E. Korty, E.M. Shevach, and T.R. Malek. 1986. J. Immunol. 137:3240-3246.
2. Palfeee, R.G.E., F.J. Dumont, and U. Hammerling. 1986. Immunogenetics 23:197-207.
3. Rock, K.L., H. Reiser, A. Bamezai, J. McGrew, and B. Benacerraf. 1989. Immunol. Rev. 111:195-224.
4. Moore, T., M. Bennett, and V. Kumar. 1995. J. Immunol. 154:1653-1663.
5. Ito, M., K. Anan, M. Masawa, S. Kai, and H. Hara. 1996. Stem Cells 14:412-418.
6. Yonemura, Y., H. Ku, S.D. Lyman, and M. Ogawa. 1997. Blood 89: 1915-1921.
7. Codias, E.K., and C. Cray, R.D. Baler, R.B. Levy, and T.R. Malek. 1989. Immunogenetics 29:98-107.
8. Malek, T.R., K.M. Danis, and E.K. Codias. 1989. J. Immunol. 142:1929-1936.
9. Codias, E.K., and T.R. Malek. 1990. J. Immunol. 144:2197-2204.
10. Malek, T.R., G. Ortega, C. Chan, R.A. Kroczek, and E.M. Shevach. 1986. J. Exp. Med. 164:709-722.
11. Codias, E.K., J.E. Rutter, T.J. Fleming, and T.R. Malek. 1990. J. Immunol. 145:1407-1414.
12. Ivanov, V., T.J. Fleming, and T.R. Malek. 1994. J. Immunol. 153:2394-2406.
13. Flood, P.M., J.P. Dougherty, and Y. Ron. 1993. J. Exp. Med. 172:115-120.
2. Palfeee, R.G.E., F.J. Dumont, and U. Hammerling. 1986. Immunogenetics 23:197-207.
3. Rock, K.L., H. Reiser, A. Bamezai, J. McGrew, and B. Benacerraf. 1989. Immunol. Rev. 111:195-224.
4. Moore, T., M. Bennett, and V. Kumar. 1995. J. Immunol. 154:1653-1663.
5. Ito, M., K. Anan, M. Masawa, S. Kai, and H. Hara. 1996. Stem Cells 14:412-418.
6. Yonemura, Y., H. Ku, S.D. Lyman, and M. Ogawa. 1997. Blood 89: 1915-1921.
7. Codias, E.K., and C. Cray, R.D. Baler, R.B. Levy, and T.R. Malek. 1989. Immunogenetics 29:98-107.
8. Malek, T.R., K.M. Danis, and E.K. Codias. 1989. J. Immunol. 142:1929-1936.
9. Codias, E.K., and T.R. Malek. 1990. J. Immunol. 144:2197-2204.
10. Malek, T.R., G. Ortega, C. Chan, R.A. Kroczek, and E.M. Shevach. 1986. J. Exp. Med. 164:709-722.
11. Codias, E.K., J.E. Rutter, T.J. Fleming, and T.R. Malek. 1990. J. Immunol. 145:1407-1414.
12. Ivanov, V., T.J. Fleming, and T.R. Malek. 1994. J. Immunol. 153:2394-2406.
13. Flood, P.M., J.P. Dougherty, and Y. Ron. 1993. J. Exp. Med. 172:115-120.
Other Notes
Small volumes of Ly-6A/E (Sca-1) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for Ly-6A/E (Sca-1). It may not necessarily be applicable to this product.
Protein Family
Lymphocyte antigen
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.