Rat IgG2a Isotype Control APC
Référence MBS1801856-0,1mg
Conditionnement : 0.1mg
Marque : MyBiosource
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IgG2a Isotype Control – Rat Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1801856MBS1801856 is an isotype control targeting IgG2a. Also known as Rat IgG2a Isotype Control APC. Reactive in Limpet. Host: Rat. More details
Product Overview
Online Datasheet in PDFProduct Name
IgG2a, Isotype Control
Full Product Name
Rat IgG2a Isotype Control APC
Product Gene Name
IgG2a isotype control
Clonality
Monoclonal
Isotype
Rat IgG2a
Clone Number
RTG2A1-1
Host
Rat
Species Reactivity
Limpet
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Purity/Purification
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Concentration
0.1mg/ml (lot specific)
Specificity
This rat IgG2a kappa monoclonal antibody (clone RTG2A1-1, also known as G2A-1-1 or KLH/G2A-1-1) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Conjugation
APC
Negative Control
The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10ug/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Applications Tested/Suitable for IgG2a isotype control
Flow Cytometry (FC/FACS) (QC tested)
Product References and Citations for IgG2a isotype control
*Berge T, Gronningsaeter IH, Lorvik KB, Abrahamsen G, Granum S, Sundvold-Gjerstad V, Corthay A, Bogen B, Spurkland A: SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice. PLoS One. 2012;7(10):e48239., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=berge+t+l,*Talkington J, Nickell SP: Role of Fc gamma receptors in triggering host cell activation and cytokine release by Borrelia burgdorferi. Infect Immun. 2001 Jan;69(1):413-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/11119532,*Gillan V, Devaney E: Regulatory T cells modulate Th2 responses induced by Brugia pahangi third-stage larvae. Infect Immun. 2005 Jul;73(7):4034-42., URL: http://www.ncbi.nlm.nih.gov/pubmed/15972491
Other Notes
Small volumes of IgG2a isotype control vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.