Rat Rap guanine nucleotide exchange factor 4, RAPGEF4 ELISA Kit
Référence MBS9325349-96
Conditionnement : 96-Strip-Wells
Marque : MyBiosource
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Rap Guanine Nucleotide Exchange Factor (GEF) 4 (RAPGEF4) ELISA Kit for Rat
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9325349Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of RAPGEF4 (hereafter termed "analyte") in… More details
Product Overview
Online Datasheet in PDFProduct Name
Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), ELISA Kit
Full Product Name
Rat Rap guanine nucleotide exchange factor 4, RAPGEF4 ELISA Kit
Product Gene Name
RAPGEF4 elisa kit
Product Synonym Names
Rat Rap guanine nucleotide exchange factor 4 (RAPGEF4) ELISA kit; CAMP-GEFII; CGEF2; EPAC2; Nbla00496; RAP guanine-nucleotide-exchange factor (GEF) 4; cAMP-regulated guanine nucleotide exchange factor II; exchange protein directly activated by cAMP 2; putative protei; Rap guanine nucleotide exchange factor (GEF) 4
Species Reactivity
Rat
Assay Type
Quantitative Sandwich
Detection Range
0.25ng/ml-8ng/ml
Sensitivity
0.1ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C
Other Notes
Small volumes of RAPGEF4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RAPGEF4 purchase
MBS9325349 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RAPGEF4. The ELISA analytical biochemical technique of the MBS9325349 kit is based on RAPGEF4 antibody-RAPGEF4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RAPGEF4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RAPGEF4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for RAPGEF4. It may not necessarily be applicable to this product.
NCBI Official Full Name
Rap guanine nucleotide exchange factor 4
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor (GEF) 4
NCBI Official Symbol
Rapgef4
NCBI Official Synonym Symbols
CAMPS; Cgef2; Epac2; CAMP-GEFII
UniProt Protein Name
Rap guanine nucleotide exchange factor 4
UniProt Synonym Protein Names
Exchange factor directly activated by cAMP 2; Exchange protein directly activated by cAMP 2; EPAC 2; cAMP-regulated guanine nucleotide exchange factor II; cAMP-GEFII
Protein Family
Rap guanine nucleotide exchange factor
UniProt Gene Name
Rapgef4
UniProt Synonym Gene Names
Cgef2; Epac2; EPAC 2; cAMP-GEFII
UniProt Entry Name
RPGF4_RAT
NCBI Protein Information
rap guanine nucleotide exchange factor 4; epac 2; exchange factor directly activated by cAMP 2; exchange protein directly activated by cAMP 2; cAMP-regulated guanine nucleotide exchange factor II
NCBI Summary for RAPGEF4
bind cAMP and activate the guanine nucleotide binding protein Rap1A [RGD, Feb 2006]
UniProt Comments for RAPGEF4
RAPGEF4: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA- independent exocytosis through interaction with RIMS2. Interacts with RIMS1 and RIMS2. Probably part of a complex with RIMS2 and GTP-activated RAB3A. Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels. 3 isoforms of the human protein are produced by alternative promoter.
Protein type: GEFs, Ras; GEFs
Cellular Component: photoreceptor inner segment; protein complex; photoreceptor outer segment; basolateral plasma membrane; axon; apical plasma membrane; cytoplasm; dendrite; plasma membrane; dendritic spine; excitatory synapse; cytosol; cAMP-dependent protein kinase complex
Molecular Function: protein binding; Ras GTPase binding; guanyl-nucleotide exchange factor activity; protein complex binding; cAMP-dependent protein kinase regulator activity; cAMP binding; Ras guanyl-nucleotide exchange factor activity
Biological Process: exocytosis; heart development; regulation of dendrite development; signal transduction; positive regulation of smooth muscle cell migration; negative regulation of synaptic transmission; calcium ion-dependent exocytosis; regulation of exocytosis; cAMP-mediated signaling; insulin secretion; small GTPase mediated signal transduction; regulation of protein kinase activity; positive regulation of GTPase activity
Show more Protein type: GEFs, Ras; GEFs
Cellular Component: photoreceptor inner segment; protein complex; photoreceptor outer segment; basolateral plasma membrane; axon; apical plasma membrane; cytoplasm; dendrite; plasma membrane; dendritic spine; excitatory synapse; cytosol; cAMP-dependent protein kinase complex
Molecular Function: protein binding; Ras GTPase binding; guanyl-nucleotide exchange factor activity; protein complex binding; cAMP-dependent protein kinase regulator activity; cAMP binding; Ras guanyl-nucleotide exchange factor activity
Biological Process: exocytosis; heart development; regulation of dendrite development; signal transduction; positive regulation of smooth muscle cell migration; negative regulation of synaptic transmission; calcium ion-dependent exocytosis; regulation of exocytosis; cAMP-mediated signaling; insulin secretion; small GTPase mediated signal transduction; regulation of protein kinase activity; positive regulation of GTPase activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with RAPGEF4 elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Adaptive Immune System Pathway antibodies | Adaptive Immune System Pathway Diagram |
| Hemostasis Pathway antibodies | Hemostasis Pathway Diagram |
| Immune System Pathway antibodies | Immune System Pathway Diagram |
| Integration Of Energy Metabolism Pathway antibodies | Integration Of Energy Metabolism Pathway Diagram |
| Integrin AlphaIIb Beta3 Signaling Pathway antibodies | Integrin AlphaIIb Beta3 Signaling Pathway Diagram |
| Integrin Cell Surface Interactions Pathway antibodies | Integrin Cell Surface Interactions Pathway Diagram |
| Leukocyte Transendothelial Migration Pathway antibodies | Leukocyte Transendothelial Migration Pathway Diagram |
| Leukocyte Transendothelial Migration Pathway antibodies | Leukocyte Transendothelial Migration Pathway Diagram |
| Metabolism Pathway antibodies | Metabolism Pathway Diagram |
| Platelet Aggregation (Plug Formation) Pathway antibodies | Platelet Aggregation (Plug Formation) Pathway Diagram |
Organs/Tissues associated with RAPGEF4 elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >13 publications with RAPGEF4 and Brain |
| Heart Antibodies | >3 publications with RAPGEF4 and Heart |
| Pituitary Gland Antibodies | >2 publications with RAPGEF4 and Pituitary Gland |
| Lung Antibodies | >1 publications with RAPGEF4 and Lung |
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