Recombinant DNA Methyltransferase 1 (DNMT1)
Référence MBS2018363-0,05mg
Conditionnement : 0.05mg
Marque : MyBiosource
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DNA Methyltransferase 1 (DNMT1) Homo Sapiens (Human) Recombinant Protein
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2018363SPECIAL PROMOTION: Get FREE Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment
MBS2018363 is a recombinant protein targeting DNMT1. Also known as Recombinant DNA Methyltransferase 1 (DNMT1). Reactive in Homo sapiens (Human). Host: E.coli. More details
Product Overview
Online Datasheet in PDFProduct Name
DNA Methyltransferase 1 (DNMT1), Recombinant Protein
Full Product Name
Recombinant DNA Methyltransferase 1 (DNMT1)
Product Gene Name
DNMT1 recombinant protein
Host
E.coli
Species Reactivity
Homo sapiens (Human)
Form/Format
PBS, pH7.4, containing 0.01% Sarcosyl, 5% Trehalose.
Purity/Purification
> 90%
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: DNMT1 (MBS2015356)
Immunogen: DNMT1 (MBS2018363)
Immunogen: DNMT1 (MBS2018363)
Matching Pairs
APC-CY7 Conjugated Antibody: DNMT1 (MBS2080996)
Immunogen: DNMT1 (MBS2018363)
Immunogen: DNMT1 (MBS2018363)
Molecular Weight
Predicted Molecular Mass: 29.6kDa
Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions.
Chromosome Location
Chromosome: 19; NC_000019.9 (10244021..10305783, complement). Location: 19p13.2
OMIM
126375
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Prokaryotic expression
Residues
Val1343~Leu1570
Tags
N-terminal His Tag
Subcellular Location
Nucleus
Traits
Freeze-dried powder
Original Concentration
400ug/mL
Predicted isoelectric point
9.7
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for DNMT1 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)
Application Notes for DNMT1 recombinant protein
(May be suitable for use in other assays to be determined by the end user.)
Other Notes
Small volumes of DNMT1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for DNMT1. It may not necessarily be applicable to this product.
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 1 isoform a
NCBI Official Synonym Full Names
DNA (cytosine-5-)-methyltransferase 1
NCBI Official Symbol
DNMT1
NCBI Official Synonym Symbols
AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 1
UniProt Synonym Protein Names
CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT
UniProt Gene Name
DNMT1
UniProt Synonym Gene Names
AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI
UniProt Entry Name
DNMT1_HUMAN
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; m.HsaI; DNA MTase HsaI; DNA methyltransferase HsaI; CXXC-type zinc finger protein 9
NCBI Summary for DNMT1
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Show more UniProt Comments for DNMT1
DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.
Protein type: Amino Acid Metabolism - cysteine and methionine; Transcription regulation; Cell development/differentiation; Methyltransferase; Methyltransferase, DNA; EC 2.1.1.37
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleoplasm; centric heterochromatin; replication fork; nucleus
Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; DNA binding; methyl-CpG binding; zinc ion binding; RNA binding; chromatin binding; transcription factor binding; DNA-methyltransferase activity
Biological Process: negative regulation of histone H3-K9 methylation; transcription, DNA-dependent; maintenance of DNA methylation; gene silencing; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; regulation of gene expression, epigenetic; regulation of cell proliferation; negative regulation of gene expression, epigenetic; DNA methylation; gene expression; DNA methylation on cytosine
Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie
Show more Protein type: Amino Acid Metabolism - cysteine and methionine; Transcription regulation; Cell development/differentiation; Methyltransferase; Methyltransferase, DNA; EC 2.1.1.37
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleoplasm; centric heterochromatin; replication fork; nucleus
Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; DNA binding; methyl-CpG binding; zinc ion binding; RNA binding; chromatin binding; transcription factor binding; DNA-methyltransferase activity
Biological Process: negative regulation of histone H3-K9 methylation; transcription, DNA-dependent; maintenance of DNA methylation; gene silencing; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; regulation of gene expression, epigenetic; regulation of cell proliferation; negative regulation of gene expression, epigenetic; DNA methylation; gene expression; DNA methylation on cytosine
Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie
Research Articles on DNMT1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with DNMT1 recombinant protein
| Reference Product | PubMed Publications |
|---|---|
| DNMT3B recombinant protein | >438 publications with DNMT1 and DNMT3B |
| MECP2 recombinant protein | >91 publications with DNMT1 and MECP2 |
| HDAC1 recombinant protein | >89 publications with DNMT1 and HDAC1 |
| DMAP1 recombinant protein | >59 publications with DNMT1 and DMAP1 |
| PCNA recombinant protein | >58 publications with DNMT1 and PCNA |
| UHRF1 recombinant protein | >57 publications with DNMT1 and UHRF1 |
| SUV39H1 recombinant protein | >28 publications with DNMT1 and SUV39H1 |
| RB1 recombinant protein | >7 publications with DNMT1 and RB1 |
| USP7 recombinant protein | >4 publications with DNMT1 and USP7 |
Pathways associated with DNMT1 recombinant protein
| Products by Pathway | Pathway Diagram |
|---|---|
| Cysteine And Methionine Metabolism Pathway antibodies | Cysteine And Methionine Metabolism Pathway Diagram |
| Cysteine And Methionine Metabolism Pathway antibodies | Cysteine And Methionine Metabolism Pathway Diagram |
| Methionine Degradation Pathway antibodies | Methionine Degradation Pathway Diagram |
| Methionine Degradation Pathway antibodies | Methionine Degradation Pathway Diagram |
| MicroRNAs In Cancer Pathway antibodies | MicroRNAs In Cancer Pathway Diagram |
| MicroRNAs In Cancer Pathway antibodies | MicroRNAs In Cancer Pathway Diagram |
| One Carbon Metabolism Pathway antibodies | One Carbon Metabolism Pathway Diagram |
| RB In Cancer Pathway antibodies | RB In Cancer Pathway Diagram |
| Regulation Of Retinoblastoma Protein Pathway antibodies | Regulation Of Retinoblastoma Protein Pathway Diagram |
| Trans-sulfuration And One Carbon Metabolism Pathway antibodies | Trans-sulfuration And One Carbon Metabolism Pathway Diagram |
Diseases associated with DNMT1 recombinant protein
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >502 publications with DNMT1 and Neoplasms |
| Carcinoma Antibodies | >179 publications with DNMT1 and Carcinoma |
| Adenocarcinoma Antibodies | >85 publications with DNMT1 and Adenocarcinoma |
| Breast Neoplasms Antibodies | >78 publications with DNMT1 and Breast Neoplasms |
| Cell Transformation, Neoplastic Antibodies | >56 publications with DNMT1 and Cell Transformation, Neoplastic |
| Lung Neoplasms Antibodies | >51 publications with DNMT1 and Lung Neoplasms |
| Liver Diseases Antibodies | >47 publications with DNMT1 and Liver Diseases |
| Disease Models, Animal Antibodies | >45 publications with DNMT1 and Disease Models, Animal |
| Liver Neoplasms Antibodies | >43 publications with DNMT1 and Liver Neoplasms |
| Carcinoma, Hepatocellular Antibodies | >38 publications with DNMT1 and Carcinoma, Hepatocellular |
Organs/Tissues associated with DNMT1 recombinant protein
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >149 publications with DNMT1 and Blood |
| Brain Antibodies | >105 publications with DNMT1 and Brain |
| Liver Antibodies | >92 publications with DNMT1 and Liver |
| Lung Antibodies | >85 publications with DNMT1 and Lung |
| Embryonic Tissue Antibodies | >42 publications with DNMT1 and Embryonic Tissue |
| Prostate Antibodies | >40 publications with DNMT1 and Prostate |
| Stomach Antibodies | >31 publications with DNMT1 and Stomach |
| Kidney Antibodies | >31 publications with DNMT1 and Kidney |
| Nerve Antibodies | >29 publications with DNMT1 and Nerve |
| Bone Antibodies | >28 publications with DNMT1 and Bone |