Recombinant Human Fibroblast Growth Factor-basic
Référence cyt-218-10ug
Conditionnement : 10ug
Marque : Prospec
Catalogue number
CYT-218
Synonyms
Prostatropin, FGF-basic, Basic FGF, HBGF-2, FGF-2, FGF-b.
Introduction
Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The HPR -binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Description
The FGF-b is purified by proprietary chromatographic techniques.
Source
Physical Appearance
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution in 20mM Tris-HCl, pH7.4 and 1M NaCl.
Solubility
Stability
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 98.0% as determined by Analysis by SDS-PAGE.
Amino acid sequence
Biological Activity
The ED50, calculated by the dose-dependant proliferation of murine balb/c 3T3 cells is <0.1ng/ml, corresponding to a specific activity of graeter than 1.0x107 Units/mg.
Safety Data Sheet
Usage
References
1.Title:Activation of the Hedgehog pathway in pilocytic astrocytomas
Publication:Neuro Oncol (2010) 12 (8): 790-798. doi: 10.1093/neuonc/noq026 First published online: March 11, 2010
Link:FGF 2 prospec publication
2.Title:Phenotype reversion in fetal human liver epithelial cells identifies the role of an intermediate meso-endodermal stage before hepatic maturation
Publication:Advance Online Publication March 4, 2008 doi: 10.1242/?jcs.019315 April 1, 2008 J Cell Sci 121, 1002-1013.
Link:FGF 2 Human prospec publication
3. Title:Embryonic Stem Cell-Derived Glial Precursors as a Vehicle for Sulfamidase Production in the MPS-IIIA Mouse Brain .
Publication:
- Cell Transplantation
- ISSN: 0963-6897
- DOI: 10.3727/096368910X498944
- Volume 19, Issue 8, pages 985-998
- Copyright © 2010 Cognizant Comm. Corp
Link:Fibroblast Growth Factor-Basic prospec publication
4.Title:Adult Human Dental Pulp Stem Cells Differentiate Toward Functionally Active Neurons Under Appropriate Environmental Cues.
Publication: Article first published online: 22 MAY 2008 DOI:10.1634/stemcells.2007-0979 Copyright © 2008 AlphaMed Press.
Link:FGF-basic prospec publication
5.Title: Successful implantation of physiologically functional bioengineered mouse internal anal sphincter
Publication: American Journal of Physiology-Gastrointestinal and Liver Physiology 299.2 (2010): G430-G439.
Link: FGF-b prospec publication
Background
FGF-2 Fibroblast Growth Factor-Basic Human Recombinant: Unraveling Its Pleiotropic Roles in Development and Disease
Abstract:
FGF-2 (Fibroblast Growth Factor-Basic), also known as Basic FGF, HBGF-2, FGF-2, or FGF-b, is a multifunctional growth factor that plays diverse roles in various biological processes, including embryonic development, tissue repair, angiogenesis, and tumorigenesis.
This research paper aims to provide a comprehensive analysis of the molecular characteristics, signaling pathways, and wide-ranging physiological functions of FGF-2. Additionally, it explores the therapeutic implications of FGF-2 in different diseases and disorders.
Synonyms such as Basic FGF, HBGF-2, FGF-2, and FGF-b associated with the protein are discussed throughout the paper to highlight their relevance in scientific literature.
Introduction:
- FGF-2, also known as Basic FGF, HBGF-2, FGF-2, or FGF-b, is a growth factor with diverse roles in development and disease. This section introduces FGF-2 and its synonyms, highlighting their significance and relevance in scientific research.
Molecular Characteristics of FGF-2:
- This section explores the molecular characteristics of FGF-2, including its primary amino acid sequence, protein structure, and post-translational modifications. The importance of these factors in determining FGF-2's biological activity and receptor binding is discussed.
Signaling Pathways Activated by FGF-2:
- FGF-2 activates specific signaling pathways upon binding to its receptors, leading to diverse cellular responses. This section focuses on the activation of the MAPK/ERK, PI3K/Akt, and PLCγ pathways. The downstream effectors and transcriptional regulators involved in mediating FGF-2's cellular responses are also discussed.
Physiological Functions of FGF-2:
- FGF-2 plays critical roles in various physiological processes, including embryonic development, tissue regeneration, angiogenesis, and wound healing. This section provides an in-depth analysis of FGF-2's contributions to these processes, emphasizing its role in cell proliferation, migration, differentiation, and extracellular matrix remodeling.
Therapeutic Implications of FGF-2:
- The multifaceted properties of FGF-2 make it a promising therapeutic candidate for various diseases and disorders. This section discusses the potential applications of FGF-2 in tissue engineering, wound healing, cardiovascular diseases, and neurodegenerative disorders. The challenges and future directions in utilizing FGF-2 as a therapeutic agent are also explored.
FGF-2 in Disease Pathogenesis:
- FGF-2 is implicated in the pathogenesis of several diseases, including cancer, fibrosis, and neurologic disorders. This section examines the role of FGF-2 in promoting tumor growth, angiogenesis, tissue fibrosis, and neuroprotection. The therapeutic implications and targeting of FGF-2 in disease management are also discussed.
Conclusion:
- FGF-2, also known as Basic FGF, HBGF-2, FGF-2, or FGF-b, is a critical growth factor involved in various biological processes and disease pathogenesis. Understanding the molecular characteristics, signaling pathways, and physiological functions of FGF-2 contributes to the exploration of its therapeutic potential in different disorders.