Recombinant Human Glycoprotein A33 overexpression cell culture supernatant, secreted from the serum-free medium of transfected HEK293 cells. Purchase will also include one vial of normal control, the culture supernatant of HEK293 cell transfected with empty expression vector.
Recombinant Human Glycoprotein A33
Recombinant Human Glycoprotein A33
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Product Description
Specifications
| Size | 200 µL |
| Species | Human |
| Accession Number | Q99795 |
| Gene Symbols | GPA33 |
| Protein Name / Synonyms | Glycoprotein A33, Glycoprotein A33 (Transmembrane), Transmembrane Glycoprotein A33, Transmembrane Glycoprotein A33, A33 |
| Expressed Region | Ile22-Val235 |
| Expression System | HEK293 cells |
| Tag | N-terminal 6x histidine tag |
| Purity | Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80°C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Molecular Weight (kDa) | Recombinant Human Glycoprotein A33 has a calculated molecular mass of 24 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs). |
| Format | Pink liquid |
| Formulation | Culture supernatant of transfected HEK293 cells |
| Preservative | None |
| Shipping Type | Blue ice |
| Storage | -20°C |
Stability & Storage
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.