Recombinant human Chorionic Gonadotropin, Beta Polypeptid/hCG-beta overexpression cell lysate, derived from the transfected HEK293 cells. (100 µg). Purchase will also include one vial of normal control HEK293 cell lysate (Catalog # 230-10006) transfected with empty expression vector.
Recombinant Human hCG-beta Protein, HEK293 Lysate
Recombinant Human hCG-beta Protein, HEK293 Lysate
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Product Description
Specifications
| Size | 100 µg |
| Species | Human |
| Accession Number | P01233 |
| Gene Symbols | CGB |
| Protein Name / Synonyms | Chorionic Gonadotropin, Beta Polypeptide, Chorionic Gonadotrophin Chain Beta, CGB3, Chorionic Gonadotropin Beta 3 Subunit, Chorionic Gonadotropin Beta Subunit, Chorionic Gonadotropin Beta Chain, Choriogonadotropin Subunit Beta, CGB CGB5 CGB7 CGB8, CGB5, CGB7, CGB8, HCGB, Choriogonadotropin subunit beta, CG-beta |
| Expressed Region | Ser21-Gln165 |
| Expression System | HEK293 cells |
| Tag | N-terminal 6x histidine tag |
| Purity | Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Molecular Weight (kDa) | Recombinant protein has a calculated molecular mass of 16. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs). |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS containing mammalian cell protease inhibitor cocktail |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Stability & Storage
Shipping
Including one vial of HEK293 cell lysate transfected with empty expression vector.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.