Recombinant Mouse Tumor Necrosis Factor/TNFalpha Protein
Référence MBS2553644-0,01mg
Conditionnement : 0.01mg
Marque : MyBiosource
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Bioactivity
Tumor Necrosis Factor/Tnfalpha (TNFalpha) Mouse Recombinant Protein
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2553644Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the… More details
Product Overview
Online Datasheet in PDFProduct Name
Tumor Necrosis Factor/TNFalpha, Recombinant Protein
Full Product Name
Recombinant Mouse Tumor Necrosis Factor/TNFalpha Protein
Product Gene Name
TNFalpha recombinant protein
Product Synonym Names
Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; Tumor Necrosis Factor; Membrane Form; Tumor Necrosis Factor; Soluble Form; Tnf; Tnfa; Tnfsf2
Host
E Coli
Form/Format
Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4.
Purity/Purification
>95% as determined by reducing SDS-PAGE.
Preparation and Storage
Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
Molecular Weight
Molecular Mass: 16.4 kDa
Actual Protein Molecular Mass: 14 kDa
Actual Protein Molecular Mass: 14 kDa
Sequence Positions
Asp89-Leu235
Species
Mouse
Endotoxin
<1.0 EU per ug as determined by LAL test.
Bio-Activity
Measured to a cytoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 2-8pg/mL.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of TNFalpha recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TNFalpha purchase
MBS2553644 is a recombinant protein targeting TNFalpha. Also known as Recombinant Mouse Tumor Necrosis Factor/TNFalpha Protein. Host: E Coli.
Additional Details
NCBI/Uniprot data below describe general gene information for TNFalpha. It may not necessarily be applicable to this product.
NCBI Official Full Name
tumor necrosis factor isoform 2
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
Tnf
NCBI Official Synonym Symbols
DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
UniProt Protein Name
Tumor necrosis factor
UniProt Synonym Protein Names
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
UniProt Gene Name
Tnf
UniProt Synonym Gene Names
Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2
NCBI Protein Information
tumor necrosis factor
NCBI Summary for TNFalpha
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Show more UniProt Comments for TNFalpha
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed:25586176).
Show more Research Articles on TNFalpha
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with TNFalpha recombinant protein
| Reference Product | PubMed Publications |
|---|---|
| Jun recombinant protein | >13741 publications with TNFalpha and Jun |
| Rela recombinant protein | >1476 publications with TNFalpha and Rela |
| Nfkbia recombinant protein | >1051 publications with TNFalpha and Nfkbia |
| Fos recombinant protein | >638 publications with TNFalpha and Fos |
| Tnfrsf1a recombinant protein | >505 publications with TNFalpha and Tnfrsf1a |
| Ripk1 recombinant protein | >243 publications with TNFalpha and Ripk1 |
| Adipoq recombinant protein | >183 publications with TNFalpha and Adipoq |
| Nfkb1 recombinant protein | >137 publications with TNFalpha and Nfkb1 |
| Slc2a4 recombinant protein | >79 publications with TNFalpha and Slc2a4 |
| Ppargc1a recombinant protein | >48 publications with TNFalpha and Ppargc1a |
Pathways associated with TNFalpha recombinant protein
| Products by Pathway | Pathway Diagram |
|---|---|
| AGE-RAGE Signaling Pathway In Diabetic Complications antibodies | AGE-RAGE Signaling Pathway In Diabetic Complications Diagram |
| AGE-RAGE Signaling Pathway In Diabetic Complications antibodies | AGE-RAGE Signaling Pathway In Diabetic Complications Diagram |
| Adipocytokine Signaling Pathway antibodies | Adipocytokine Signaling Pathway Diagram |
| Adipocytokine Signaling Pathway antibodies | Adipocytokine Signaling Pathway Diagram |
| Adipogenesis Pathway antibodies | Adipogenesis Pathway Diagram |
| African Trypanosomiasis Pathway antibodies | African Trypanosomiasis Pathway Diagram |
| African Trypanosomiasis Pathway antibodies | African Trypanosomiasis Pathway Diagram |
| Allograft Rejection Pathway antibodies | Allograft Rejection Pathway Diagram |
| Allograft Rejection Pathway antibodies | Allograft Rejection Pathway Diagram |
| Alzheimer's Disease Pathway antibodies | Alzheimer's Disease Pathway Diagram |
Diseases associated with TNFalpha recombinant protein
| Disease Name | Pubmed Publications |
|---|---|
| Necrosis Antibodies | >97871 publications with TNFalpha and Necrosis |
| Inflammation Antibodies | >37322 publications with TNFalpha and Inflammation |
| Lung Diseases Antibodies | >7457 publications with TNFalpha and Lung Diseases |
| Liver Diseases Antibodies | >5734 publications with TNFalpha and Liver Diseases |
| Insulin Resistance Antibodies | >2830 publications with TNFalpha and Insulin Resistance |
| Fibrosis Antibodies | >2808 publications with TNFalpha and Fibrosis |
| Colitis Antibodies | >2791 publications with TNFalpha and Colitis |
| Atherosclerosis Antibodies | >2679 publications with TNFalpha and Atherosclerosis |
| Pain Antibodies | >2599 publications with TNFalpha and Pain |
| Reperfusion Injury Antibodies | >2412 publications with TNFalpha and Reperfusion Injury |
Organs/Tissues associated with TNFalpha recombinant protein
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Skin Antibodies | >5339 publications with TNFalpha and Skin |
| Joint Antibodies | >4971 publications with TNFalpha and Joint |
| Bone Marrow Antibodies | >4478 publications with TNFalpha and Bone Marrow |
| Spleen Antibodies | >4396 publications with TNFalpha and Spleen |
| Lymph Node Antibodies | >2083 publications with TNFalpha and Lymph Node |
| Embryonic Tissue Antibodies | >1750 publications with TNFalpha and Embryonic Tissue |
| Thymus Antibodies | >915 publications with TNFalpha and Thymus |
| Prostate Antibodies | >878 publications with TNFalpha and Prostate |
| Mammary Gland Antibodies | >230 publications with TNFalpha and Mammary Gland |
| Uncharacterized Tissue Antibodies | >18 publications with TNFalpha and Uncharacterized Tissue |