Serum Triglyceride Quantification Kit (Colorimetric)

Background: Triglycerides (TAG) are a type of lipid in the blood, serving as an energy source and playing a key role in metabolism. Triglycerides are the digestive end product of breaking down dietary fats. Any extra carbohydrates and fats that are not immediately used are chemically converted into triglycerides. In the intestines, secreted enzyme lipases hydrolyse the triglyceride ester bond, yielding glycerol and free fatty acids in a process called lipolysis. Enterocytes then absorb and repackage the fragments with cholesterol to form chylomicrons, a major lipoprotein transport particle. In the liver, hepatic lipases also break down triglycerides to assemble another lipoprotein particle (VLDL) from triglycerides, cholesterol, and apolipoproteins. Serum Triglyceride Quantification Kit measures triglyceride concentration in serum, plasma, and lysates by a coupled enzymatic reaction system. First, lipase hydrolyzes the triglyceride ester bond, yielding glycerol. The glycerol is then phosphorylated and oxidized, producing hydrogen peroxide which reacts with the kit's Colorimetric Probe (absorbance maxima of 570 nm). The Serum Triglyceride Quantification Kit is a simple, colorimetric assay that quantitatively measures the amount of triglyceride in plasma, serum, and lysates in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, triglyceride standards, free glycerol controls and unknown samples. The kit contains a triglyceride standard and has a detection sensitivity limit of ~10 uM (1 mg/dL).

1. Bucolo, G. and David, H. (1973) Clin. Chem. 19(5), 476-482.
2. Fossati, P. and Prencipe, L. (1982) Clin. Chem. 28(10), 2077-2080.
3. McGowan, M.W. et al. (1983) Clin. Chem. 29(3), 538-542.
4. Mendez, A.J., Cabeza, C., and Hsia, S.L. (1986) Anal. Biochem. 156, 386-389.
1. Chellan, B. et al. (2014). IL-22 is induced by S100/calgranulin and impairs cholesterol efflux in macrophages by downregulating AGCB1. J. Lipid Res. 55:443-454.
2. Marino, A. et al. (2014). ITCH Deficiency Protects From Diet-Induced Obesity. Diabetes. 63:550-561.

Small volumes of Serum Triglyceride assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS168769 is an assay kit targeting Serum Triglyceride. Also known as Serum Triglyceride Quantification Kit (Colorimetric).