This kit isolates total DNA from 180–220 mg of fresh or frozen soil samples. Once solubilized, genomic DNA from plants, animals, viruses, bacteria, and parasites binds to a nucleic acid purification spin column while degraded proteins and PCR inhibitors are filtered out. Following wash steps with Buffers WB and RP, pure genomic DNA is eluted with Buffer TE for immediate use in downstream molecular assays.