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> T-box 1 (Tbx1, T-box family of transcription factors)

T-box 1 (Tbx1, T-box family of transcription factors)

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Référence T1848-24-100ug

Conditionnement : 100ug

Marque : US Biological

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T1848-24 T-box 1 (Tbx1, T-box family of transcription factors)

Clone Type
Polyclonal
Host
rabbit
Source
mouse
Swiss Prot
P70323
Isotype
IgG
Grade
Affinity Purified
Applications
IHC IP WB
Crossreactivity
Mo
Gene #
Tbx1
Shipping Temp
Blue Ice
Storage Temp
-20°C

T-box 1 (or Tbx1) is a member of a gene family characterized by the presence of a region known as the T-box, which is a DNA-binding domain homologous to a region found in the protein product of the T gene. The T, or Brachyury, gene was first identified as being responsible for a mutated mouse phenotype with a short, blunt-ended tail. The T-box DNA-binding domain is 173-185 amino acids in length, and demonstrates strong evolutionary conservation across vertebrate species. Seven mouse T-box genes have been identified: Tbx1-Tbx6 and Tbr-1. Tbx1 gene expression has been studied by RTPCR and in situ hybridization (ISH) throughout murine embryonic development; high expression levels correspond with cranial neural crest cell migration (embryonic days 8.5 – 10.5) and therefore suggest an important role in the development of facial and glandular structures of the head and neck region, including the parathyroid and thymus glands. The human ortholog of the mouse Tbx1 gene has been mapped to chromosome region. a region commonly deleted in DiGeorge syndrome (DGS). In humans, DGS is characterized by conotruncal cardiac defects, aplasia or hyperplasia of the thymus and parathyroid glands, palate abnormalities, developmental delay, and craniofacial dysmorphia. Mouse models of heterozygous null Tbx1 mutations produce cardiovascular defects, disrupted neural crest and cranial nerve migration, middle and inner ear defects, and developmental abnormalities, modeling the human DiGeorge syndrome.

Applications:
Suitable for use in Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:20
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: ~0.25mg/ml|Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Synthetic peptide corresponding to the internal region of the mouse T-box 1 (Tbx1) protein. Species sequence homology: three isoforms (A, B, C) of human T-box 1: 98%.|Specificity: Recognizes mouse T-box 1 protein. On Western blots, a ~50kD band is identified in mouse testis, lung, and skeletal muscle homogenates. In mouse testis, an additional band at ~40kD, which may represent an isoform of Tbox 1, is also identified. Reactivity has been confirmed with mouse testis, lung, and skeletal muscle tissue homogenates.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to the internal region of the mouse T-box 1 (Tbx1) protein. Species sequence homology: three isoforms (A, B, C) of human T-box 1: 98%.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse T-box 1 protein. On Western blots, a ~50kD band is identified in mouse testis, lung, and skeletal muscle homogenates. In mouse testis, an additional band at ~40kD, which may represent an isoform of Tbox 1, is also identified. Reactivity has been confirmed with mouse testis, lung, and skeletal muscle tissue homogenates.
References
1. Bollag RJ, et al. Nat Genet 7:383-389, 1994. 2. Bulfone A, et al. Neuron 15:63-78, 1995. 3. Chapman DL, et al. Developmental Dynamics 206:379-390, 1996. 4. Chieffo C, et al. Genomics 43:267-277, 1997. 5. Lindsay EA, et al. Nature 410:97-101, 2001. 6. Jerome LA, Papaioannou VE. Nat Genet 27(3):286-291, 2001. 7. Epstein JA. Trends Genet 17(10):S13-17, 2001. 8. Funke B, et al. Hum Mol Genet 10(22):2549-2556, 2001. 9. Vitelli F, et al. Hum Mol Genet 11(8):915-922, 2002.