TF siRNA (Rat)
Référence MBS829280-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
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TF siRNA – Serotransferrin (Rat)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829280MBS829280 is an siRNA targeting TF. Also known as TF siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit TF expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
TF, siRNA
Full Product Name
TF siRNA (Rat)
Product Gene Name
TF sirna
Product Synonym Names
Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Liver regeneration-related protein LRRG03; Siderophilin
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
TF siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
54,524 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for TF sirna
RNA Interference (RNAi)
Other Notes
Small volumes of TF sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for TF. It may not necessarily be applicable to this product.
NCBI Official Full Name
serotransferrin
NCBI Official Synonym Full Names
transferrin
NCBI Official Symbol
Tf
NCBI Official Synonym Symbols
Tfn; Trf
UniProt Protein Name
Serotransferrin
UniProt Synonym Protein Names
Beta-1 metal-binding globulin; Liver regeneration-related protein LRRG03; Siderophilin
Protein Family
TFIIA-alpha and beta-like factor
UniProt Gene Name
Tf
UniProt Synonym Gene Names
Transferrin
UniProt Entry Name
TRFE_RAT
NCBI Protein Information
serotransferrin
NCBI Summary for TF
plays a role iin iron transport and homeostasis [RGD, Feb 2006]
UniProt Comments for TF
TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; cell surface; early endosome; cytoplasmic membrane-bound vesicle; coated pit; recycling endosome; endocytic vesicle; perinuclear region of cytoplasm; late endosome; apical plasma membrane; basal plasma membrane; basement membrane; endosome; vesicle
Molecular Function: GTP binding; ferric iron binding; ferric iron transmembrane transporter activity
Biological Process: positive regulation of myelination; cellular iron ion homeostasis; apoptosis; positive regulation of transcription, DNA-dependent; actin filament organization; osteoclast differentiation; response to organic cyclic substance; iron ion transport; activation of JNK activity; retinal homeostasis; small GTPase mediated signal transduction; response to lead ion; response to hypoxia; acute-phase response; positive regulation of bone resorption; ferric iron transport; positive regulation of phosphorylation
Show more Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; cell surface; early endosome; cytoplasmic membrane-bound vesicle; coated pit; recycling endosome; endocytic vesicle; perinuclear region of cytoplasm; late endosome; apical plasma membrane; basal plasma membrane; basement membrane; endosome; vesicle
Molecular Function: GTP binding; ferric iron binding; ferric iron transmembrane transporter activity
Biological Process: positive regulation of myelination; cellular iron ion homeostasis; apoptosis; positive regulation of transcription, DNA-dependent; actin filament organization; osteoclast differentiation; response to organic cyclic substance; iron ion transport; activation of JNK activity; retinal homeostasis; small GTPase mediated signal transduction; response to lead ion; response to hypoxia; acute-phase response; positive regulation of bone resorption; ferric iron transport; positive regulation of phosphorylation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with TF sirna
| Reference Product | PubMed Publications |
|---|---|
| Tfr2 sirna | >44 publications with TF and Tfr2 |
| Tfrc sirna | >22 publications with TF and Tfrc |
| Slc40a1 sirna | >7 publications with TF and Slc40a1 |
| Apoa1 sirna | >5 publications with TF and Apoa1 |
| Heph sirna | >5 publications with TF and Heph |
| Steap3 sirna | >2 publications with TF and Steap3 |
Pathways associated with TF sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| HIF-1 Signaling Pathway antibodies | HIF-1 Signaling Pathway Diagram |
| Hemostasis Pathway antibodies | Hemostasis Pathway Diagram |
| Iron Uptake And Transport Pathway antibodies | Iron Uptake And Transport Pathway Diagram |
| Mineral Absorption Pathway antibodies | Mineral Absorption Pathway Diagram |
| Mineral Absorption Pathway antibodies | Mineral Absorption Pathway Diagram |
| Platelet Activation, Signaling And Aggregation Pathway antibodies | Platelet Activation, Signaling And Aggregation Pathway Diagram |
| Platelet Degranulation Pathway antibodies | Platelet Degranulation Pathway Diagram |
| Relationship Between Glutathione And NADPH Pathway antibodies | Relationship Between Glutathione And NADPH Pathway Diagram |
| Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies | Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram |
| Transferrin Endocytosis And Recycling Pathway antibodies | Transferrin Endocytosis And Recycling Pathway Diagram |
Diseases associated with TF sirna
| Disease Name | Pubmed Publications |
|---|---|
| Cardiovascular Diseases Antibodies | >1541 publications with TF and Cardiovascular Diseases |
| Inflammation Antibodies | >848 publications with TF and Inflammation |
| Nervous System Diseases Antibodies | >717 publications with TF and Nervous System Diseases |
| Carcinoma Antibodies | >551 publications with TF and Carcinoma |
| Necrosis Antibodies | >529 publications with TF and Necrosis |
| Immune System Diseases Antibodies | >444 publications with TF and Immune System Diseases |
| Atherosclerosis Antibodies | >300 publications with TF and Atherosclerosis |
| Liver Diseases Antibodies | >262 publications with TF and Liver Diseases |
| Lung Neoplasms Antibodies | >187 publications with TF and Lung Neoplasms |
| Liver Neoplasms Antibodies | >147 publications with TF and Liver Neoplasms |
Organs/Tissues associated with TF sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >1003 publications with TF and Brain |
| Liver Antibodies | >660 publications with TF and Liver |
| Lung Antibodies | >502 publications with TF and Lung |
| Kidney Antibodies | >460 publications with TF and Kidney |
| Embryonic Tissue Antibodies | >388 publications with TF and Embryonic Tissue |
| Eye Antibodies | >199 publications with TF and Eye |
| Colon Antibodies | >145 publications with TF and Colon |
| Placenta Antibodies | >127 publications with TF and Placenta |
| Spleen Antibodies | >122 publications with TF and Spleen |
| Connective Tissue Antibodies | >119 publications with TF and Connective Tissue |