TK1 siRNA (Rat)
Référence MBS828745-30nmol
Conditionnement : 30nmol
Marque : MyBiosource
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TK1 siRNA – Thymidine Kinase, Cytosolic (Rat)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828745MBS828745 is an siRNA targeting TK1. Also known as TK1 siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit TK1 expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
TK1, siRNA
Full Product Name
TK1 siRNA (Rat)
Product Gene Name
TK1 sirna
Product Synonym Names
TK-1; Thymidine kinase. cytosolic; Fragment
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
TK1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
13,511 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for TK1 sirna
RNA Interference (RNAi)
Other Notes
Small volumes of TK1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for TK1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
thymidine kinase, cytosolic
NCBI Official Synonym Full Names
thymidine kinase 1, soluble
NCBI Official Symbol
Tk1
NCBI Official Synonym Symbols
Tk
UniProt Protein Name
Thymidine kinase, cytosolic
Protein Family
Thymidine kinase
UniProt Gene Name
Tk1
UniProt Synonym Gene Names
Tk-1
UniProt Entry Name
KITH_RAT
NCBI Protein Information
thymidine kinase, cytosolic
NCBI Summary for TK1
catalyzes the transfer of the terminal phosphate of ATP to thymidine to form thymidine 5'-phosphate (dTMP) [RGD, Feb 2006]
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with TK1 sirna
| Reference Product | PubMed Publications |
|---|---|
| Tyms sirna | >5 publications with TK1 and Tyms |
| Birc5 sirna | >3 publications with TK1 and Birc5 |
| Cdk1 sirna | >2 publications with TK1 and Cdk1 |
| Dctd sirna | >2 publications with TK1 and Dctd |
| Tymp sirna | >2 publications with TK1 and Tymp |
| Dut sirna | >1 publications with TK1 and Dut |
Pathways associated with TK1 sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Drug Metabolism - Other Enzymes Pathway antibodies | Drug Metabolism - Other Enzymes Pathway Diagram |
| Drug Metabolism - Other Enzymes Pathway antibodies | Drug Metabolism - Other Enzymes Pathway Diagram |
| Metabolic Pathways antibodies | Metabolic Pathways Diagram |
| Metabolism Pathway antibodies | Metabolism Pathway Diagram |
| Metabolism Of Nucleotides Pathway antibodies | Metabolism Of Nucleotides Pathway Diagram |
| Pyrimidine Metabolism Pathway antibodies | Pyrimidine Metabolism Pathway Diagram |
| Pyrimidine Metabolism Pathway antibodies | Pyrimidine Metabolism Pathway Diagram |
| Pyrimidine Metabolism Pathway antibodies | Pyrimidine Metabolism Pathway Diagram |
| Pyrimidine Salvage Reactions Pathway antibodies | Pyrimidine Salvage Reactions Pathway Diagram |
Diseases associated with TK1 sirna
| Disease Name | Pubmed Publications |
|---|---|
| Breast Neoplasms Antibodies | >31 publications with TK1 and Breast Neoplasms |
| Neoplasms, Experimental Antibodies | >26 publications with TK1 and Neoplasms, Experimental |
| Lung Neoplasms Antibodies | >26 publications with TK1 and Lung Neoplasms |
| Lung Diseases Antibodies | >22 publications with TK1 and Lung Diseases |
| Adenocarcinoma Antibodies | >19 publications with TK1 and Adenocarcinoma |
| Disease Models, Animal Antibodies | >15 publications with TK1 and Disease Models, Animal |
| Colorectal Neoplasms Antibodies | >15 publications with TK1 and Colorectal Neoplasms |
| Brain Diseases Antibodies | >10 publications with TK1 and Brain Diseases |
| Nervous System Diseases Antibodies | >9 publications with TK1 and Nervous System Diseases |
| Neoplasm Metastasis Antibodies | >9 publications with TK1 and Neoplasm Metastasis |
Organs/Tissues associated with TK1 sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Lung Antibodies | >33 publications with TK1 and Lung |
| Liver Antibodies | >20 publications with TK1 and Liver |
| Prostate Antibodies | >9 publications with TK1 and Prostate |
| Kidney Antibodies | >7 publications with TK1 and Kidney |
| Embryonic Tissue Antibodies | >6 publications with TK1 and Embryonic Tissue |
| Heart Antibodies | >6 publications with TK1 and Heart |
| Connective Tissue Antibodies | >4 publications with TK1 and Connective Tissue |
| Pancreas Antibodies | >3 publications with TK1 and Pancreas |
| Thymus Antibodies | >3 publications with TK1 and Thymus |
| Placenta Antibodies | >1 publications with TK1 and Placenta |