TLR4 ELISA Kit (Rat)
Référence OKEH00569
Conditionnement : 96Wells
Marque : Aviva Systems Biology
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TLR4 ELISA Kit (Rat) : 96 Wells (OKEH00569)
| Datasheets/Manuals | Printable datasheet for TLR4 ELISA Kit (Rat) : 96 Wells (OKEH00569) |
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| COA Datasheet | Printable COA datasheet for OKEH00569 |
| Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology TLR4 ELISA Kit (Rat) (OKEH00569) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TLR4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TLR4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TLR4 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.8% (n = 20) Mean Inter-assay CV%: < 8.1% (n = 20) | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 0.159 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Rat Toll-like receptor 4 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Tlr4 |
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| Gene Full Name | toll-like receptor 4 |
| Alias Symbols | toll-like receptor 4, toll4. |
| NCBI Gene Id | 29260 |
| Protein Name | Toll-like receptor 4 |
| Description of Target | Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (By similarity). |
| Uniprot ID | Q9QX05 |
| Protein Accession # | NP_003257.1 |
| Nucleotide Accession # | NM_003266.3 |
| Protein Size (# AA) | 839 |
| Molecular Weight | 95 kDa |
