TMB Membrane Substrate

Optimal conditions to be determined by end user. The product should be allowed to equilibrate to room temperature (25 degree C) prior to use. For use, completely cover the membrane surface with substrate. The substrate system will react with sites on the membrane containing peroxidase producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. The reaction should be monitored and read before the background color becomes to intense which may result in diminished contrast between the positive and background staining. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.