TNF Alpha (Biotin)

TNF alpha is produced by activated macrophages, monocytes, endothelial cells, fibroblast and both T and B lymphocytes. Production of ovine TNF alpha can be induced by a variety of factors, dependant upon cell type, and includes bacterial toxins, IL-1, PDGF, IFN beta, NGF, oncostatin M and viral infections. TNF alpha functions in the regulation of immune cells, is able to induce fever, apoptotic cell death, sepsis, cacheria, inflammation and to inhibit tumorigenesis and viral replication.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 0.05-0.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.