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> WC1 (FITC)

WC1 (FITC)

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Référence W0481-74F-100ug

Conditionnement : 100ug

Marque : US Biological

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W0481-74FWC1 (FITC)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcesheep
Swiss ProtQ9BDF1
IsotypeIgG1
GradeAffinity Purified
ApplicationsFC
CrossreactivityBo Gt Sh
Shipping TempBlue Ice
Storage Temp-20°C

WC1 cell surface antigen is a ~215kD glycoprotein, expressed by a population of gamma/delta T lymphocytes (Wijngaard et al.1992).

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS-1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 10F582|Host: mouse|Source: sheep|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Sheep thymocytes.|Specificity: Recognizes sheep WC1. Species Crossreactivity: bovine, goat.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Sheep thymocytes.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes sheep WC1. Species Crossreactivity: bovine, goat.
References
1. Mackay, C. et al. (1986) Three distinct subpopulations of sheep T lymphocytes. Eur. J. Immunol. 16: 19 - 25. 2. Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset. Cell. Immunol. 110: 46 -55.