GH1 ELISA Kit (Salmon)

Référence OKEH00068

Conditionnement : 96Wells

Marque : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivitySalmon
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GH1 ELISA Kit (Salmon) (OKEH00068) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-salmon GH1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GH1 and incubated. The GH1 found in the sample or standards competes with the biotinylated GH1 for limited binding sites on the immobilized anti-salmon GH1 antibody. Excess unbound biotinylated GH1 and sample or standard GH1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GH1 captured in the well and inversely proportional to the amount of GH1 which was contained in the sample or standard.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.9% (n = 20)
Mean Inter-assay CV%: < 9.8% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-GH1 Microplate96 Wells (12 x 8 Well strips)
GH1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X GH1-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.245 ng/mL
SpecificityNatural and recombinant Salmon Somatotropin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolGH1
Gene Full Namegrowth hormone prepeptide
Alias Symbolsgh
NCBI Gene Id100136588
Protein NameSomatotropin-1
Description of TargetGrowth hormone plays an important role in growth control and is involved in the regulation of several anabolic processes. Implicated as an osmoregulatory substance important for seawater adaptation.
Uniprot IDQ91222
Protein Accession #NP_001117148
Nucleotide Accession #NM_001123676
Protein Size (# AA)210
Molecular Weight24 kDa

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