PRMT5, Chemiluminescent BioAssay™ Kit (Protein Arginine Methyltransferase 5, HRMT1L5, IBP72, JBP1, SKB1)

PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3.

Suitable for studying enzyme kinetics and HTS applications.

The PRMT5 Chemiluminescent BioAssay™ Kit is designed to measure PRMT5 activity for screening and profiling applications. The PRMT5 Chemiluminescent BioAssay™ Kit comes in a convenient format, with a 384-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine3 residue of Histone H4, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified PRMT5 enzyme for 384 enzyme reactions. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

DMSO >1%, strong acids or bases, ionic detergents, high salt

PRMT5/MEP50 human enzyme, 1x40ug
100uM S-adenosylmethionine, 1x250ul
Primary antibody 4-3, 1x100ul
Secondary HRP-labeled antibody 2, 2x10ul
4x PRMT5 assay buffer (add DTT before experiment), 2x3ml
Blocking buffer, 2x50ml
HRP chemiluminescent substrate A (transparent bottle), 2x6ml
HRP chemiluminescent substrate B (brown bottle), 2x6ml
384-well plate precoated with histone substrate, 1x plate

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.