PRMT8, Chemiluminescent BioAssay™ Kit (Protein Arginine N-methyltransferase 8, Histone-arginine N-methyltransferase 8, HRMT1L3, HRMT1L4)
Référence 377020-96T
Conditionnement : 96Tests
Marque : US Biological
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
Shipping Temp
Blue IceStorage Temp
4°C/-20°C/-70°CIntended Use:
Suitable for studying enzyme kinetics and HTS applications.
Test Principle:
The PRMT8 Chemiluminescent BioAssay™ Kit is designed to measure PRMT8 activity for screening and profiling applications. The PRMT8 Chemiluminescent BioAssay™ Kit comes in a convenient format with a 96-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine 3 (R3) residue of Histone H4, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified PRMT8 enzyme for 100 enzyme reactions. The key to the PRMT8 Chemiluminescent BioAssay™ Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S- adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt
Kit Components:
PRMT8 His-FLAG-tags, 1x10ug
20uM S-adenosylmethionine*, 1x250ul
Primary antibody 4, 1x100ul
Secondary HRP-labeled antibody 2, 1x10ul
4x HMT assay buffer 2, 1x3ml
Blocking buffer, 1x50ml
HRP chemiluminescent substrate A (transparent bottle), 1x6ml
HRP chemiluminescent substrate B (brown bottle), 1x6ml
96-well plate precoated with histone substrate, 1x plate
* Decreasing the S-adenosylmethionine concentration will make the assay more sensitive to test inhibitors.
20uM S-adenosylmethionine*, 1x250ul
Primary antibody 4, 1x100ul
Secondary HRP-labeled antibody 2, 1x10ul
4x HMT assay buffer 2, 1x3ml
Blocking buffer, 1x50ml
HRP chemiluminescent substrate A (transparent bottle), 1x6ml
HRP chemiluminescent substrate B (brown bottle), 1x6ml
96-well plate precoated with histone substrate, 1x plate
* Decreasing the S-adenosylmethionine concentration will make the assay more sensitive to test inhibitors.
Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.