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> Blockout Universal Blocking Buffer For Western Blotting

Blockout Universal Blocking Buffer For Western Blotting

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Référence orb348645

Conditionnement : 3x500ml

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Blockout Universal Blocking Buffer For Western Blotting

Blockout Universal Blocking Buffer For Western Blotting

Catalog Number: orb348645

Catalog Numberorb348645
CategoryProteins
DescriptionBlockout Universal Blocking Buffer For Western Blotting
Tested applicationsWB
Concentration1X
Dilution rangeWB: User Defined
Form/AppearanceLiquid (sterile filtered)
PurityBlocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
ConjugationUnconjugated
Hazard InformationNon-Toxic
StorageStore Blocking Buffer at 4° C prior to opening. DO NOT FREEZE.
Buffer/PreservativesThimerosal is added as an antimicrobial agent.
Alternative namesMultiplex Blocking Buffer, Immunoblot Blocking Buf
NoteFor research use only
Application notesBlockOut allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and other applications making it a convenient general purpose blocking agent. Antibody conjugates prepared with IRDye 800 and IRDye 700DX (Licor), Cy2, Cy3, Cy3.5, Cy5 and Cy5.5 (GE Healthcare), DyLight405, DyLight 549, DyLight 649, DyLight 680, and DyLight 800 (Thermo Fisher/Pierce) and Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 647 and Alexa Fluor 680 (Invitrogen/Molecular Probes) and ATTO (Atto-Tec) have been validated on various platforms using this product with superior results compared to other commercially available products. In the infrared range, where little to no autofluorescence occurs, specific signal is sharply evident from any background giving the best possible signal-to-noise ratio. This allows for detection levels in the picogram range which rivals the sensitivity of chemiluminescence on film for western blotting. Superior results are also seen when this product is used for simultaneous labeling (multiplex) in western blots or microscopy using various fluorochrome combinations for multicolor imaging. Membranes blocked with BlockOut can be dried and are very stable.
Expiration Date6 months from date of receipt.

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