4E Binding Protein 1 (Eukaryotic Translation Initiation Factor 4E Binding Protein 1, eIF4E Binding Protein 1, eIF4EBP1, 4EBP1, 4E-BP1, BP-1, MGC4316, Phosphorylated Heat- and Acid-stable Protein Regulated by Insulin 1, PHAS-1, PHAS I, PHAS-I) [KO Validated]

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the eIF4E translation initiation factor. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity. Multiple 4E-BP1 residues are phosphorylated in vivo. While phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (IC): 1:800-1:3200
Flow Cytometry (fixed/permeabilized): 1:1600
Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:1200-1:4800
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.