Reagents and instruments for immunology, cell biology and molecular biology.
 
> 4E Binding Protein 1 (Eukaryotic Translation Initiation Factor 4E Binding Protein 1, eIF4E Binding Protein 1, eIF4EBP1, 4EBP1, 4E-BP1, BP-1, MGC4316, Phosphorylated Heat- and Acid-stable Protein Regulated by Insulin 1, PHAS-1, PHAS I, PHAS-I) [KO Validated]

4E Binding Protein 1 (Eukaryotic Translation Initiation Factor 4E Binding Protein 1, eIF4E Binding Protein 1, eIF4EBP1, 4EBP1, 4E-BP1, BP-1, MGC4316, Phosphorylated Heat- and Acid-stable Protein Regulated by Insulin 1, PHAS-1, PHAS I, PHAS-I) [KO Validated]

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4E Binding Protein 1 (Eukaryotic Translation Initiation Factor 4E Binding Protein 1, eIF4E Binding Protein 1, eIF4EBP1, 4EBP1, 4E-BP1, BP-1, MGC4316, Phosphorylated Heat- and Acid-stable Protein Regulated by Insulin 1, PHAS-1, PHAS I, PHAS-I) [KO Validated]

Cat# 0004-21A-100ul

Size : 100ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

0004-21A4E Binding Protein 1 (Eukaryotic Translation Initiation Factor 4E Binding Protein 1, eIF4E Binding Protein 1, eIF4EBP1, 4EBP1, 4E-BP1, BP-1, MGC4316, Phosphorylated Heat- and Acid-stable Protein Regulated by Insulin 1, PHAS-1, PHAS I, PHAS-I) [KO Validated]

Specifications
Clone TypeMonoclonal
Hostrabbit
Sourcehuman
Swiss ProtQ13541
IsotypeIgG
GradePurified
ApplicationsFC IC IF IHC IP WB KO
CrossreactivityHu Mk Mo Rt
Shipping TempBlue Ice
Storage Temp-20°C

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the eIF4E translation initiation factor. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity. Multiple 4E-BP1 residues are phosphorylated in vivo. While phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (IC): 1:800-1:3200
Flow Cytometry (fixed/permeabilized): 1:1600
Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:1200-1:4800
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG|Clone No: 10g124 (53H11)|Host: rabbit|Source: human|Concentration: Not Determined|Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.|Purity: Purified|Immunogen: Synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1.|Specificity: Recognizes endogenous levels of total human 4E-BP1 protein. Species Crossreactivity: monkey, mouse and rat.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified
Specificity
Recognizes endogenous levels of total human 4E-BP1 protein. Species Crossreactivity: monkey, mouse and rat.
References
1. Pause, A. et al. (1994) Nature 371, 762-7.|2. Brunn, G.J. et al. (1997) Science 277, 99-101.|3. Gingras, A.C. et al. (1998) Genes Dev 12, 502-13.|4. Fadden, P. et al. (1997) J Biol Chem 272, 10240-7.|5. Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.