OARA01739 | Anti-STREPTAVIDIN Antibody Biovalley

Anti-STREPTAVIDIN Antibody (OARA01739)

Datasheets/Manuals	Printable datasheet for Anti-STREPTAVIDIN Antibody (OARA01739)

Product Info
Predicted Species Reactivity	Streptavidin
Product Format	Lyophilized. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Clonality	Polyclonal
Host	Rabbit
Conjugation	Unconjugated
Application	DB\|ELISA\|IF\|IHC\|WB
Additional Information	Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
::	Preservative: 0.01% (w/v) Sodium Azide Stabilizer: None
Reconstitution and Storage	-20°C or -80°C\|2°C to 8°C
Immunogen	Streptavidin (Streptomyces avidinii)
Purification	Anti-STREPTAVIDIN antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Streptavidin.
Predicted Homology Based on Immunogen Sequence	Streptomyces avidinii
Concentration	70 mg/ml
Specificity	Anti-streptavidin antibody detects Streptavidin, a 60 kDa protein purified from the bacterium Streptomyces avidinii. No cross reactivity occurs against Avidin.
Application Info	Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. ELISA: 1:20,000-1:100,000 WB: 1:2,000-1:10,000 IHC: 1:1,000-1:5,000

Description of Target	Streptavidin is a homo-tetrameric protein having a very high affinity for biotin (vitamin B7). Streptavidin bound biotin has a dissociation constant (Kd) on the order of about 10^-14 mol/L. Streptavidin and biotin are used extensively in molecular biology and bionanotechnology due to the resistance of streptavidin-biotin complex to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH.