Conditionnement : 50mg
Téléphone : +1 850 650 7790
Synonyms: Solid Green O, Solid Green Crystals O
| Description | Malachite green (Solid Green Crystals O) is an N-methylated diaminotriphenylmethane dye used for coloring. |
| Synonyms | Solid Green O, Solid Green Crystals O |
| Cell Research | Instructions I. Solution preparation: 1. Preparation of storage solution: Dissolve Safranin O in deionized water to prepare a 0.1%-1% (w/v) stock solution. If a higher solubility is required, a small amount of ethanol can be added to help dissolve. Note: The stock solution should be stored in a dark place and refrigerated to avoid dye degradation. 2. Preparation of working solution: Dilute the storage solution to the required working solution according to experimental requirements. II. Biological tissue staining 1. Sample fixation: Fix the tissue sample in formalin solution, dehydrate and embed. After slicing, prepare the tissue slices for staining. 2. Staining steps: 1) Place the tissue slices in Safranin O staining solution. The staining time is adjusted according to the sample type, generally 2-5 minutes. 2) If contrast staining is required, Fast Green or other suitable dyes can be used. 3) Use 70%-95% ethanol for graded decolorization until the dye in non-target areas is removed. 4) Sealing: Seal the slide with neutral gum or other suitable sealing agent to prevent the sample from drying or dye loss. 5) Microscopic observation: Use an optical microscope to observe the staining results: Cell nuclei and cartilage matrix will appear red. Collagen fibers, extracellular matrix, etc. will appear green (if Fast Green is used as a contrast dye). Precautions: 1) Operation safety: Wear gloves and goggles when using to avoid contact of dye with skin or eyes. 2) Dye storage: Safranin O is light-sensitive and should be stored in a dark place and refrigerated. Ensure that there is no precipitation in the dye before use. 3) Contrast staining: Select a suitable contrast stain (such as Fast Green) according to the purpose of the experiment. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Molecular Weight | 364.91 |
| Formula | C23H25ClN2 |
| Cas No. | 569-64-2 |
| Smiles | [Cl-].CN(C)c1ccc(cc1)C(=C1C=CC(C=C1)=[N+](C)C)c1ccccc1 |
| Relative Density. | 1.0448 g/cm3 (Estimated) |
| Storage | Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 125 mg/mL (342.55 mM), Sonication is recommended.  | |||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+90% Saline: 10 mg/mL (27.4 mM), Solution. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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