RayBio® Human BMP-7 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human BMP-7 ELISA Kit
Human BMP-7 ELISA Kit
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 10 pg/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 10 pg/ml - 6000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, BMP4, BMP-6, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GMCSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, MMP-2, MMP-3, MMP-9, MMP-10, MMP-13, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P18075 |
| Gene Id | 655 |
| Gene Symbols | BMP7, OP1 |
| Protein Name / Synonyms | Bone morphogenetic protein 7 (BMP-7) (Osteogenic protein 1) (OP-1) (Eptotermin alfa) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease, TGF-beta Signaling |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
citations
Functional Characterization of Renal Tubular Epithelial Cells in Optimized Coculture Systems
- Species:
- Human
- Sample Type:
- proximal tubule epithelial cells (RPTECs), bone marrow mesenchymal stem cells (BMMSCs)
- Year:
- 2023
- Journal Name:
- Nephron
The use of heparin/polycation coacervate sustain release system to compare the bone regenerative potentials of 5 BMPs using a critical sized calvarial bone defect model
- Species:
- Human
- Sample Type:
- muscle-derived stem cells (MDSCs)
- Year:
- 2022
- Journal Name:
- Biomaterials
Are bone morphogenetic protein-7 (BMP-7) serum levels correlated with development of hepatic fibrosis J Infect Dev Ctries 2014; 8(5):605-610.
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2014
- Journal Name:
- The Journal of Infection in Developing Countries
Serum concentration of bone morphogenetic proteins(BMPs) is not linked to serum anti-mullerian hormone(AMH) level
- Species:
- Human
- Sample Type:
- Serum (anti-mullerian hormone patient detection)
- Year:
- 2014
Auricular cartilage repair using cryogel scaffolds loaded with BMP7expressing primary chondrocytes.
- Species:
- Rabbit
- Sample Type:
- Conditioned Media (Auricular Chondrocytes)
- Year:
- 2013
- PubMed ID:
- 23281155
Bone morphogenetic protein-7 and disease progression in renal amyloidosis patients.
- Species:
- Human
- Sample Type:
- Serum (chronic renal disease patients)
- Year:
- 2013
- PubMed ID:
- 23902471
A nanoparticulate injectable hydrogel as a tissue engineering scaffold for multiple growth factor delivery for bone regeneration
- Species:
- not specified
- Sample Type:
- Nanomaterials (monitoring concentration in a nanoparticle encapsulation experiment)
- Year:
- 2012
- Journal Name:
- International Journal of Nanomedicine
Bone morphogenetic protein-7 is a MYC target with prosurvival functions in childhood medulloblastoma.
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2011
- Journal Name:
- Oncogene
- PubMed ID:
- 21317922
Effluent markers related to epithelial mesenchymal transition with adjusted values for effluent cancer antigen 125 in peritoneal dialysis patients.
- Species:
- Human
- Sample Type:
- Effluent (peritineal dialysis effluent)
- Year:
- 2011
- Journal Name:
- International journal of nephrology
- PubMed ID:
- 21755056
Acidic preparations of lysed platelets upregulate proliferative pathways in osteoblast-like cells as demonstrated by genome-wide microarray analysis.
- Species:
- Human
- Sample Type:
- Cell Lysate (platelets)
- Year:
- 2011
- PubMed ID:
- 21740339
