RayBio® Human EGF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human EGF ELISA Kit
Human EGF ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 1 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1 pg/ml - 200 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 5 - 50 fold |
| Accession Number | P01133 |
| Gene Id | 1950 |
| Gene Symbols | EGF |
| Protein Name / Synonyms | Pro-epidermal growth factor (EGF) [Cleaved into: Epidermal growth factor (Urogastrone)] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
citations
Gelatin-grafted tubular asymmetric scaffolds promote ureteral regeneration via activation of the integrin/Erk signaling pathway
- Species:
- Human
- Sample Type:
- human uroepithelial cells (HUC)
- Year:
- 2023
- Journal Name:
- Frontiers in bioengineering and biotechnology
Surface roughness modulates EGFR signaling and stemness of triple-negative breast cancer cells
- Species:
- Human
- Sample Type:
- MDA-MB-231 cell, MDA-MB-468 cell
- Year:
- 2023
- Journal Name:
- Frontiers in cell and developmental biology
Preparation of redispersible liposomal dry powder using an ultrasonic spray freeze-drying technique for transdermal delivery of human epithelial growth factor
- Year:
- 2023
- Journal Name:
- International Journal of Nanomedicine
GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands
- Species:
- Human
- Sample Type:
- Conditioned Media (Tumor xenografts)
- Year:
- 2016
- Journal Name:
- Molecular Cancer
- PubMed ID:
- 26586721
In vitro and ex vivo characterization of nanonized amniotic membrane particles: An untapped modality for ocular surface reconstruction
- Species:
- Human
- Sample Type:
- Amniotic Membrane (AM)
- Year:
- 2023
- Journal Name:
- Experimental Eye Research
Conditioned Medium Originated From Lethally Irradiated Umbilical Cord Blood-Derived Mononuclear Cells Has Different Pro-Angiogenic Effects Over Mature and Progenitor Endothelial Cells In Vitro.
- Species:
- Human
- Sample Type:
- Conditioned Media (monnuclear cells)
- Year:
- 2013
- Journal Name:
- Blood
Erlotinib prolongs survival in pancreatic cancer by blocking gemcitabine-induced MAPK signals
- Species:
- Human
- Sample Type:
- Cell Lysate (pancreatic ductal carcinoma)
- Year:
- 2013
- Journal Name:
- Cancer Research
- PubMed ID:
- 23378339
In vivo fluorescence imaging for cancer diagnosis using receptor-targeted epidermal growth factor-based nanoprobe.
- Species:
- Human
- Sample Type:
- Nanomaterials (human EGF and EGF-NP w)
- Year:
- 2013
- Journal Name:
- Biomaterials
- PubMed ID:
- 23998858
Platelet Microparticles Enhance the Vasoregenerative Potential of Angiogenic Early Outgrowth Cells After Vascular Injury
- Species:
- Human
- Sample Type:
- Conditioned Media (HUVEC cells in vascular injury model)
- Year:
- 2010
- Journal Name:
- Circulation
- PubMed ID:
- 20644015
Specific nanomarkers fluorescence: in vitro analysis for EGFR overexpressed cells in triple-negative breast cancer and malignant glioblastoma
- Species:
- Human
- Sample Type:
- MDA-MB-468 cell, M059J cell
- Year:
- 2022
- Journal Name:
- Photodiagnosis and Photodynamic Therapy
