RayBio® Human HGF ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human HGF ELISA Kit
Human HGF ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 3 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 3 pg/ml - 2000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P14210 |
| Gene Id | 3082 |
| Gene Symbols | HGF, HPTA |
| Protein Name / Synonyms | Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain Hepatocyte growth factor beta chain] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation, Autophagy, Antibody Drug Development, Protein Therapeutics |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
An optimized procedure for preparation of conditioned medium from Wharton’s jelly mesenchymal stromal cells isolated from umbilical cord
- Species:
- Human
- Sample Type:
- mesenchymal stromal cells (MSCs)
- Year:
- 2023
- Journal Name:
- Frontiers in molecular biosciences
Human adipose-derived mesenchymal stem cells laden in gellan gum spongy-like hydrogels for volumetric muscle loss treatment
- Species:
- Human
- Sample Type:
- human adipose-derived mesenchymal stem cells (hASCs)
- Year:
- 2023
- Journal Name:
- Biomedical Materials
Mutant KRAS modulates colorectal cancer cells invasive response to fibroblast-secreted factors through the HGF/C-MET axis
- Species:
- Human
- Sample Type:
- HCT116 cell
- Year:
- 2022
- Journal Name:
- International Journal of Cancer
Modulation of Fibroblast Phenotype by Colorectal Cancer Cell-Secreted Factors Is Mostly Independent of Oncogenic KRAS
- Species:
- Human
- Sample Type:
- HCT116 cell, LS174T cell
- Year:
- 2022
- Journal Name:
- Cells
Development of Porous Hydrogel Scaffolds with Multiple Cues for Liver Tissue Engineering.
- Species:
- Human
- Sample Type:
- Conditioned Media (PLGA nanospheres); Nanospheres (3D scaffold with growth factor-encapsulated nanospheres)
- Year:
- 2017
- Journal Name:
- Regenerative Engineering and Translational Medicine
Serum concentrations of HGF and IL-8 in patients with active Graves' orbitopathy before and after methylprednisolone therapy
- Species:
- Human
- Sample Type:
- Serum (Graves Disease Patients. )
- Year:
- 2016
- Journal Name:
- Journal of Endocrinological Investigation
- PubMed ID:
- 26062519
Glucocorticoids and the non-steroidal selective glucocorticoid receptor modulator, compound A, differentially affect colon cancer-derived myofibroblasts
- Species:
- Human
- Sample Type:
- Conditioned Media (CT5.hTERT myofibroblasts )
- Year:
- 2015
- Journal Name:
- The Journal of Steroid Biochemistry and Molecular Biology
- PubMed ID:
- 25666906
Shed Syndecan-1 Translocates to the Nucleus of Cells Delivering Growth Factors and Inhibiting Histone Acetylation: A NOVEL MECHANISM OF TUMOR-HOST CROSS-TALK
- Species:
- Human
- Sample Type:
- Conditioned Media (CAG myeloma cells)
- Year:
- 2015
- PubMed ID:
- 25404732
Everolimus immunosuppression reduces the serum expression of fibrosis markers in liver transplant recipients
- Species:
- Human
- Sample Type:
- Serum (Liver Transplant patients)
- Year:
- 2014
- Journal Name:
- World journal of transplantation
Angiogenic properties of dehydrated human amnion/chorion allografts
- Species:
- Human
- Sample Type:
- Tissue Lysate (Dehydrated Human Amnion/Chorion Membrane Allografts)
- Year:
- 2014
- Journal Name:
- Vascular Cell
