Human Lactate Dehydrogenase A (LDHA) ELISA Kit
Référence MBS2023923-96
Conditionnement : 96-Strip-Wells
Marque : MyBiosource
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Lactate Dehydrogenase A (LDHA) ELISA Kit for Human
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2023923SPECIAL PROMOTION: Get FREE Starbucks gift card ( for 48-Wells-Kit or for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to LDHA. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to LDHA. Next, Avidin… More details
Product Overview
Online Datasheet in PDFProduct Name
Lactate Dehydrogenase A (LDHA), ELISA Kit
Full Product Name
Human Lactate Dehydrogenase A (LDHA) ELISA Kit
Product Gene Name
LDHA elisa kit
Product Synonym Names
LDH-A; LDH-M; LDH1; PIG19; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; Renal carcinoma antigen NY-REN-59
Species Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Human serum, plasma, tissue homogenates and other biological fluids
Detection Range
78-5,000pg/mL
Sensitivity
< 27pg/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of LDHA. No significant cross-reactivity or interference between LDHA and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LDHA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LDHA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
26,712 Da
OMIM
150000
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Categories/Family for LDHA elisa kit
Enzyme & Kinase; Metabolic pathway; Cardiovascular biology; Hepatology
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Other Notes
Small volumes of LDHA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for LDHA purchase
MBS2023923 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lactate Dehydrogenase A (LDHA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LDHA. The ELISA analytical biochemical technique of the MBS2023923 kit is based on LDHA antibody-LDHA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LDHA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LDHA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for LDHA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI Official Full Name
lactate dehydrogenase A, partial
UniProt Protein Name
L-lactate dehydrogenase A chain
UniProt Synonym Protein Names
Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59
Protein Family
L-lactate dehydrogenase
UniProt Gene Name
LDHA
UniProt Synonym Gene Names
LDH-A; LDH-M
UniProt Entry Name
LDHA_HUMAN
UniProt Comments for LDHA
LDH-A: Homotetramer. Belongs to the LDH/MDH superfamily. LDH family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; Apoptosis; EC 1.1.1.27; Oxidoreductase; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - cysteine and methionine
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: membrane; mitochondrion; cytosol; nucleus
Molecular Function: protein binding; L-lactate dehydrogenase activity
Biological Process: cellular metabolic process; substantia nigra development; glycolysis; cellular response to extracellular stimulus; cellular carbohydrate metabolic process; pyruvate metabolic process
Disease: Glycogen Storage Disease Xi
Show more Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; Apoptosis; EC 1.1.1.27; Oxidoreductase; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - cysteine and methionine
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: membrane; mitochondrion; cytosol; nucleus
Molecular Function: protein binding; L-lactate dehydrogenase activity
Biological Process: cellular metabolic process; substantia nigra development; glycolysis; cellular response to extracellular stimulus; cellular carbohydrate metabolic process; pyruvate metabolic process
Disease: Glycogen Storage Disease Xi
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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