Human Occludin (OCLN) ELISA Kit
Référence MBS2024381-96
Conditionnement : 96-Strip-Wells
Marque : MyBiosource
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Occludin (OCLN) ELISA Kit for Human
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Popular Catalog #: MBS2024381SPECIAL PROMOTION: Get FREE Starbucks gift card ( for 48-Wells-Kit or for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to OCLN. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to OCLN. Next, Avidin… More details
Product Overview
Online Datasheet in PDFProduct Name
Occludin (OCLN), ELISA Kit
Full Product Name
Human Occludin (OCLN) ELISA Kit
Product Gene Name
OCLN elisa kit
Product Synonym Names
Tight Junction Protein Occludin TM4 Minus
Species Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Human tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.059ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of OCLN. No significant cross-reactivity or interference between OCLN and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level OCLN were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level OCLN were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
8,175 Da
OMIM
251290
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Categories/Family for OCLN elisa kit
Signal Transduction
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Other Notes
Small volumes of OCLN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for OCLN purchase
MBS2024381 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Occludin (OCLN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OCLN. The ELISA analytical biochemical technique of the MBS2024381 kit is based on OCLN antibody-OCLN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OCLN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OCLN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for OCLN. It may not necessarily be applicable to this product.
NCBI Official Full Name
occludin
NCBI Official Synonym Full Names
occludin
NCBI Official Symbol
OCLN
NCBI Official Synonym Symbols
BLCPMG; PPP1R115
UniProt Protein Name
Occludin
Protein Family
Occludin
UniProt Gene Name
OCLN
UniProt Entry Name
OCLN_HUMAN
NCBI Protein Information
occludin
NCBI Summary for OCLN
This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
Show more UniProt Comments for OCLN
occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 5q13.1
Cellular Component: tight junction; endocytic vesicle; apicolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; cytoplasmic vesicle; intercellular junction; cytosol; cell junction
Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity
Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; protein complex assembly; cell structure disassembly during apoptosis
Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria
Show more Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 5q13.1
Cellular Component: tight junction; endocytic vesicle; apicolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; cytoplasmic vesicle; intercellular junction; cytosol; cell junction
Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity
Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; protein complex assembly; cell structure disassembly during apoptosis
Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with OCLN elisa kit
| Reference Product | PubMed Publications |
|---|---|
| TJP1 elisa kit | >527 publications with OCLN and TJP1 |
| CLDN1 elisa kit | >275 publications with OCLN and CLDN1 |
| CLDN5 elisa kit | >131 publications with OCLN and CLDN5 |
| CLDN4 elisa kit | >64 publications with OCLN and CLDN4 |
| CLDN3 elisa kit | >48 publications with OCLN and CLDN3 |
| TJP2 elisa kit | >48 publications with OCLN and TJP2 |
| CLDN7 elisa kit | >17 publications with OCLN and CLDN7 |
| TJP3 elisa kit | >14 publications with OCLN and TJP3 |
| CGN elisa kit | >7 publications with OCLN and CGN |
| YES1 elisa kit | >1 publications with OCLN and YES1 |
Pathways associated with OCLN elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Apoptosis Pathway antibodies | Apoptosis Pathway Diagram |
| Apoptotic Cleavage Of Cell Adhesion Proteins Pathway antibodies | Apoptotic Cleavage Of Cell Adhesion Proteins Pathway Diagram |
| Apoptotic Cleavage Of Cellular Proteins Pathway antibodies | Apoptotic Cleavage Of Cellular Proteins Pathway Diagram |
| Apoptotic Execution Phase Pathway antibodies | Apoptotic Execution Phase Pathway Diagram |
| Cell Adhesion Molecules (CAMs) Pathway antibodies | Cell Adhesion Molecules (CAMs) Pathway Diagram |
| Cell Adhesion Molecules (CAMs) Pathway antibodies | Cell Adhesion Molecules (CAMs) Pathway Diagram |
| Hepatitis C Pathway antibodies | Hepatitis C Pathway Diagram |
| Hepatitis C Pathway antibodies | Hepatitis C Pathway Diagram |
| Leukocyte Transendothelial Migration Pathway antibodies | Leukocyte Transendothelial Migration Pathway Diagram |
| Leukocyte Transendothelial Migration Pathway antibodies | Leukocyte Transendothelial Migration Pathway Diagram |
Diseases associated with OCLN elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Inflammation Antibodies | >140 publications with OCLN and Inflammation |
| Disease Models, Animal Antibodies | >118 publications with OCLN and Disease Models, Animal |
| Necrosis Antibodies | >82 publications with OCLN and Necrosis |
| Cardiovascular Diseases Antibodies | >81 publications with OCLN and Cardiovascular Diseases |
| Edema Antibodies | >60 publications with OCLN and Edema |
| Liver Diseases Antibodies | >43 publications with OCLN and Liver Diseases |
| Retinal Diseases Antibodies | >33 publications with OCLN and Retinal Diseases |
| Colitis Antibodies | >29 publications with OCLN and Colitis |
| Kidney Diseases Antibodies | >15 publications with OCLN and Kidney Diseases |
| Hemorrhage Antibodies | >14 publications with OCLN and Hemorrhage |
Organs/Tissues associated with OCLN elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >267 publications with OCLN and Brain |
| Intestine Antibodies | >231 publications with OCLN and Intestine |
| Liver Antibodies | >121 publications with OCLN and Liver |
| Eye Antibodies | >102 publications with OCLN and Eye |
| Kidney Antibodies | >71 publications with OCLN and Kidney |
| Testis Antibodies | >56 publications with OCLN and Testis |
| Embryonic Tissue Antibodies | >52 publications with OCLN and Embryonic Tissue |
| Lung Antibodies | >47 publications with OCLN and Lung |
| Stomach Antibodies | >19 publications with OCLN and Stomach |
| Pancreas Antibodies | >15 publications with OCLN and Pancreas |
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