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Human Resistin ELISA Kit
Human Resistin ELISA Kit
RayBio® Human Resistin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 2 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 2 pg/ml - 400 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 20 - 200 fold |
| Accession Number | Q9HD89 |
| Gene Id | 56729 |
| Gene Symbols | RETN, FIZZ3, HXCP1, RSTN, UNQ407/PRO1199 |
| Protein Name / Synonyms | Resistin (Adipose tissue-specific secretory factor) (ADSF) (C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein) (Cysteine-rich secreted protein A12-alpha-like 2) (Cysteine-rich secreted protein FIZZ3) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Human Resistin qIPCR ELISA Kit
As low as $749.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human Resistin ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Resistin (RETN), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Periodontitis in Patients With Severe Obesity: From the Oral and Gut Microbiota Dysregulation to the Visceral Adipose Tissue Inflammatory and Metabolic Disorders
- Species:
- Human
- Sample Type:
- Plasma; Tissue Lysate / Homogenate (adipose tissue protein lysates); Saliva
- Year:
- 2026
- Journal Name:
- FASEB Journal
- PubMed ID:
- 42037322
Mitochondrial and Redox Changes in Periodontitis and Type 2 Diabetes Human Blood Mononuclear Cells
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Antioxidants
Resistin, Elastase, and Lactoferrin as Potential Plasma Biomarkers of Pediatric Inflammatory Bowel Disease Based on Comprehensive Proteomic Screens
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Molecular & Cellular Proteomics
Synovial fluid but not plasma interleukin-8 is associated with clinical severity and inflammatory markers in knee osteoarthritis women with joint effusion
- Species:
- Human
- Sample Type:
- Other Body Fluid (synovial fluid); Plasma
- Year:
- 2021
- Journal Name:
- Scientific Reports
- PubMed ID:
- 33664374
Obesity as a modifying factor of periodontal therapy outcomes: local and systemic adipocytokines and oxidative stress markers
- Species:
- Human
- Sample Type:
- gingival crevicular fluid (GCF), Serum
- Year:
- 2023
- Journal Name:
- Clinical Oral Investigations
Resistin Is Increased in Periodontal Cells and Tissues: In Vitro and In Vivo Studies
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (cell-free supernatants of PDL cells)
- Year:
- 2020
- Journal Name:
- Mediators of Inflammation
- PubMed ID:
- 32410876
Altered resistin and IL6 in Neonatal sepsis in patients admitted in a tertiary care teaching hospital at Eastern India
- Species:
- Human
- Sample Type:
- Serum (neonatal sepsis cases and controls)
- Year:
- 2020
- Journal Name:
- International Journal of Health and Clinical Research
Prophylactic effect of rectal indomethacin plus nitroglycerin administration for preventing pancreatitis after endoscopic retrograde cholangiopancreatography in female patients
- Species:
- Human
- Sample Type:
- Serum (female ERCP patients)
- Year:
- 2020
- Journal Name:
- Annals of Palliative Medicine
- PubMed ID:
- 33302662
Oral contraceptive use increases risk of inflammatory and coagulatory disorders in women with Polycystic Ovarian Syndrome: An observational study
- Species:
- Human
- Sample Type:
- Whole Blood (PCOS women, OC users and non-users)
- Year:
- 2019
- Journal Name:
- Scientific Reports
- PubMed ID:
- 31308416
Altered gut-liver axis and hepatic adiponectin expression in OSAS: novel mediators of liver injury in paediatric non-alcoholic fatty liver
- Species:
- Human
- Sample Type:
- Serum (Biomarkers in pediatric non-alcoholic fatty liver patients)
- Year:
- 2015
- Journal Name:
- Thorax
- PubMed ID:
- 26069285
