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Human SPARC (Osteonectin) ELISA Kit
Human SPARC (Osteonectin) ELISA Kit
RayBio® Human SPARC (Osteonectin) ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 0.11 ng/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.11 ng/ml - 31.25 ng/ml |
| Specificity | This ELISA antibody pair detects Human SPARC. Other species not determined. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 80 fold |
| Accession Number | P09486 |
| Gene Id | 6678 |
| Gene Symbols | ON, SPARC |
| Protein Name / Synonyms | SPARC (Basement-membrane protein 40) (BM-40) (Osteonectin) (ON) (Secreted protein acidic and rich in cysteine) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Related Products
NexaTag® Human SPARC (Osteonectin) qIPCR ELISA Kit
As low as $779.00
RayPlex® Human SPARC Bead Assay
As low as $240.00
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human SPARC (Osteonectin) ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified SPARC (Osteonectin), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Exploring the Role of Serum Osteonectin and Hsp27 in Pediatric MAFLD Diagnosis and Cardiometabolic Health
- Species:
- Human
- Sample Type:
- Serum (pediatric MAFLD patients and controls)
- Year:
- 2024
- Journal Name:
- Nutrients
- PubMed ID:
- 38542777
Correlations between biomarkers of senescent cell accumulation at the systemic, tissue and cellular levels in elderly patients
- Species:
- Human
- Sample Type:
- fibroblasts (FB), mesenchymal stem/stromal cells (MSC)
- Year:
- 2023
- Journal Name:
- Experimental Gerontology
SPARC Overexpression Promotes Liver Cancer Cell Proliferation and Tumor Growth
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2021
- Journal Name:
- Frontiers in Molecular Biosciences
- PubMed ID:
- 34912848
